The new aspects of carcinoembryonic antigen as the tumor marker

• Project/Area Number: 26830100
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Tumor diagnostics
• Research Institution: Keio University (2015-2016); Shizuoka Cancer Center Research Institute (2014)
• Principal Investigator: Nakao Kanako 慶應義塾大学, 医学部(信濃町), 特任助教 (30583059)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: CEA / 腫瘍マーカー / アイソフォーム / スプライスバリアント / 膵臓がん / 癌 / 膵臓 / 膵疾患

Outline of Final Research Achievements

Carcinoembryonic antigen (CEA) is an oncofetal cell surface glycoprotein overexpressed in human gastrointestinal tumors. Because of its high expression in tumors and secretion to serum, CEA has been widely used as a serum tumor marker. However, the secretion of CEA in cancer tissues is not well defined. We previously identified two novel CEA protein isoforms in gastrointestinal cancer cell lines. CEA isoforms were also secreted to culture medium. Although CEA isoforms always co-existed with the wild type of CEA, the secretion patterns of these isoforms were different from the expression patterns. In this study, we established the stable cell lines expressing each CEAs and examined the isoforms-specific antibodies. Furthermore, we also determined the expression/secretion manner of the CEAs in pancreatic cancer cell lines.

Research Products

• [Journal Article] A novel splice variant of XIAP-associated factor 1 (XAF1) is expressed in peripheral blood containing gastric cancer-derived circulating tumor cells (2015)
  • Author(s): Hatakeyama, K., Yamakawa, Y., Fukuda, Y., Ohshima, K., Wakabayashi-Nakao, K., Sakura, N., Tanizawa, Y., Kinugasa, Y., Yamaguchi, K., Terashima, M., Mochizuki, T
  • Journal Title: Gastric Cancer Volume: 18 Issue: 4 Pages: 751-761
  • DOI: 10.1007/s10120-014-0426-3
  • Peer Reviewed
• [Journal Article] Carcinoembryonic antigen-related cell adhesion molecule 4 (CEACAM4) is specifically expressed in medullary thyroid carcinoma cells (2014)
  • Author(s): Wakabayashi-Nakao K, Hatakeyama K, Ohshima K, Ken Yamaguchi K, Mochizuki T.
  • Journal Title: Biomed Res. Volume: 35(4) Pages: 237-242
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Exosome-mediated extracellular release of polyadenylate-binding protein 1 in human metastatic duodenal cancer cells. (2014)
  • Author(s): Ohshima K, Kanto K, Hatakeyama K, Ide T, Wakabayashi-Nakao K, Watanabe Y, Sakura N, Terashima M, Yamaguchi K, Mochizuki T.
  • Journal Title: Proteomics Volume: 14 Issue: 20 Pages: 2297-2306
  • DOI: 10.1002/pmic.201300477
  • Peer Reviewed / Open Access
• [Journal Article] A novel splice variant of XIAP-associated factor 1 (XAF1) is expressed in peripheral blood containing gastric cancer-derived circulating tumor cells. (2014)
  • Author(s): Hatakeyama, K.; Yamakawa, Y.; Fukuda, Y.; Ohshima, K.; Wakabayashi-Nakao, K.; Sakura, N.; Tanizawa, Y.; Kinugasa, Y.; Yamaguchi, K.; Terashima, M.; Mochizuki, T.
  • Journal Title: Gastric cancer Volume: 印刷中
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Characterization of CFTR mutations found in Japanese cystic fibrosis patients (2017)
  • Author(s): Kanako Nakao, Reona Ishikura, Miyuki Nakakuki, Hiroshi Ishiguro, Tomoko Masaike, Yoshiro Sohma
  • Organizer: 第94回日本生理学会大会
  • Place of Presentation: アクトシティ浜松（静岡県浜松市）
  • Year and Date: 2017-03-28
• [Presentation] Characterization of G970-T1122del CFTR mutation found in Japanese CF patients (2016)
  • Author(s): Kanako Nakao, Miyuki Nakakuki, Hiroshi Ishiguro, Yoshiro Sohma
  • Organizer: 第47階日本膵臓学会大会・第20回国際膵臓学会・第6回アジアオセアニア膵臓学会
  • Place of Presentation: 仙台国際センター（宮城県仙台市）
  • Year and Date: 2016-08-04
  • Int'l Joint Research
• [Presentation] 新規CEAアイソフォームたんぱく質の生物学的意義の検証 (2014)
  • Author(s): 中尾香菜子、畠山慶一、大島啓一、山口建、望月徹
  • Organizer: 第73回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜(神奈川)
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] 分子間ジスルフィド結合の形成とN結合型糖鎖付加が起こらないヒトABCG2タンパク質は細胞に薬剤耐性を付与することができる。 (2014)
  • Author(s): 佐竹一紘、中尾香菜子、石川智久、中川大
  • Organizer: 第73回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜(神奈川)
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] 新規スプライシングバリアントXAF1は、胃がん患者末梢血中で発現している (2014)
  • Author(s): 畠山慶一、山川雄士、大島啓一、中尾香菜子、谷澤豊、絹笠祐介、山口建、寺島雅典、望月徹
  • Organizer: 第73回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜(神奈川)
  • Year and Date: 2014-09-25 – 2014-09-27
• [Patent(Industrial Property Rights)] 抗プログルカゴン抗体 (2014)
  • Inventor(s): 望月徹、大島啓一、畠山慶一、中尾香菜子、山口建
  • Industrial Property Rights Holder: 望月徹、大島啓一、畠山慶一、中尾香菜子、山口建
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2014-134688
  • Filing Date: 2014-06-30