| Project/Area Number |
26840005
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Molecular biology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
OHIRA TAKAYUKI 東京大学, 工学(系)研究科(研究院), 助教 (90727520)
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| Research Collaborator |
TSUTOMU SUZUKI 東京大学, 大学院工学系研究科, 教授 (20292782)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | tRNA / tRNA maturation / 5' capping / nuclear export / RNA modification / 成熟化 / 5'キャップ / 出芽酵母 |
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| Outline of Final Research Achievements |
We explored novel transporters required for tRNA maturation via alternative pathway in budding yeast, in which tRNA precursors (pre-tRNAs) with immature ends are exported to the cytoplasm for splicing and then imported back to nucleus to be end-matured, and identified the exportin which was required for normal tRNA maturation. In addition, we also analyzed the biosynthetic mechanism and the physiological function of 5'-capping formed at the 5'-termini of pre-tRNAs with 5'-leader sequences (pre-tRNA capping), and revealed that which was formed by a canonical capping machinery and stabilized the pre-tRNAs by preventing 5'-exonucleolytic degradation, indicating that pre-tRNA capping supported the tRNA maturation.
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