Screening of quadruplex-forming anti-prion nucleic acids and molecular design on the basis of its mechanism
Project/Area Number |
26840020
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Structural biochemistry
|
Research Institution | Kyoto University |
Principal Investigator |
Mashima Tsukasa 京都大学, エネルギー理工学研究所, 助教 (20707426)
|
Project Period (FY) |
2014-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
Keywords | アプタマー / 四重鎖核酸 / プリオン病 / 立体構造 / NMR |
Outline of Final Research Achievements |
Prion diseases typified by the spongiform encephalopathy (BSE) are thought to be caused by the accumulation of abnormal form of prion protein (PrPSc). In this study, we have performed screening of the quadruplex-forming nucleic acids that reduce PrPSc levels in prion-infected cells. We have succeeded in obtaining an RNA molecule whose IC50 value is around 100 nM. Unfortunately, NMR spectra of this RNA were too complex to analyze on account of the poor dispersion of signals. Therefore, we carried out the modification of sequence of RNA and the investigation of optimal buffer condition for NMR measurement. As a result, we found a mutated RNA molecule showing good dispersion of NMR signals and also established the suitable buffer condition for NMR measurement.
|
Report
(3 results)
Research Products
(19 results)
-
[Journal Article] Arid5a regulates naive CD4+ T cell fate through selective stabilization of Stat3 mRNA2016
Author(s)
Masuda, K., Ripley, B., Nyati, K., Dubey, P., Zaman, M., Hanieh, H., Hoga, M., Yamashita, K., Standley, D., Mashima, T., Katahira, M., Okamoto, T., Matsuura, Y., Takeuchi, O. and Kishimoto, T.
-
Journal Title
Journal of Experimental Medicine
Volume: 213
Issue: 4
Pages: 605-619
DOI
Related Report
Peer Reviewed / Open Access
-
[Journal Article] The binding specificity of translocated in liposarcoma/fused in sarcoma with lncRNA transcribed from the promoter region of cyclin D12016
Author(s)
Yoneda, R., Suzuki, S., Mashima, T., Kondo, K., Nagata, T., Katahira, M. and Kurokawa, R.
-
Journal Title
Cell Biosci.
Volume: 6(4)
Issue: 1
Pages: 1-12
DOI
NAID
Related Report
Peer Reviewed / Open Access
-
-
-
-
-
-
[Presentation] Development of the selective crosslinking reactions to 8-oxoguanine2015
Author(s)
Nagatsugi, F., Kusano, S., Ishiyama, S., Lam, S. L., Mashima, T. and Katahira, M.
Organizer
The 42nd International Symposium on Nucleic Acids Chemistry
Place of Presentation
Egret Himeji, Himeji-shi, Hyogo
Year and Date
2015-09-23
Related Report
Int'l Joint Research
-
[Presentation] NMR study of the recognition of non-coding RNA and DNA by TLS/FUS, a key regulator of cyclin D12015
Author(s)
Kondo, K., Mashima, T., Oyoshi, T., Kurokawa, R., Nagata, T. and Katahira, M.
Organizer
The 42nd International Symposium on Nucleic Acids Chemistry
Place of Presentation
Egret Himeji, Himeji-shi, Hyogo
Year and Date
2015-09-23
Related Report
Int'l Joint Research
-
-
-
-
-
-
[Presentation] Real-time NMR monitoring of enzymatic reaction of anti-HIV protein, structure of anti-prion RNA aptamer and wood biomass analysis2014
Author(s)
Kanba, K., Mashima, T., Okamura, H., Furukawa, A., Sugase, K., Nishimura, H., Watanabe, T., Nagata, T., and Katahira, M.
Organizer
The 5th Japan-Taiwan NMR symposium
Place of Presentation
Hokkaido University, Hokkaido
Year and Date
2014-09-29 – 2014-09-30
Related Report
Invited
-
-
-
-