The role of membrane curvature in the regulation of novel signaling complex

• Project/Area Number: 26840037
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Functional biochemistry
• Research Institution: Kumamoto University
• Principal Investigator: Sakamoto Yasuhisa 熊本大学, 生命科学研究部, 助教 (20613392)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 細胞膜カーブ / エンドサイトーシス / ユビキチン化

Outline of Final Research Achievements

Nedd4L is a member of HECT type E3 ubiquitin ligase Nedd4 family. Nedd4L induces endocytosis through the ubiquitination of cargo proteins. In this study, we examined the mechanism by which alpha-arrestin family ARRDC1 regulates Nedd4L. ARRDC1 directly binds to membrane curvature and recruits Nedd4L. ARRDC1 activates Nedd4L and mediates the ubiquitination of cargo protein in membrane curvature dependent manner. These results indicate that membrane curvature plays a pivotal role in the regulation of ARRDC1-Nedd4L protein complex.