Comprehensive study of structure and dynamics of laminopathy-causing LMNA mutants for elucidation of Laminopathy
| Project/Area Number |
26840050
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biophysics
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | NMR / 核ラミン |
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| Outline of Final Research Achievements |
In order to demonstrate how structural and physicochemical changes of Lamin A(LMNA) are induced by laminopathy-causing mutations, we investigated tertiary structures and dynamics of LMNA Ig-like domain by NMR by developing the “MagRO-FLYA-CYANA” system for fully-automated NMR assignment and structure determination. As a result, we determined the tertiary structures of 5 mutants of the LMNA Ig-like domain. These mutants cause different types of laminopathy.
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Report
(3 results)
Research Products
(9 results)
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[Journal Article] Physicochemical Nature of Interfaces Controlling Ferredoxin NADP+ Reductase Activity through Its Interprotein Interactions with Ferredoxin2015
Author(s)
Kinoshita Misaki, Kim Ju Yaen, Kume Satoshi, Sakakibara Yukiko, Sugiki Toshihiko, Kojima Chojiro, Kurisu Genji, Ikegami Takahisa, Hase Toshiharu, Kimata-Ariga Yoko, Lee Young-Ho
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Journal Title
Biochimica et Biophysica Acta-Bioenergetics
Volume: 1847
Issue: 10
Pages: 1200-1211
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] The crystal structure of the plant small GTPase OsRacl reveals its mode of binding to NADPH oxidase.2015
Author(s)
Kosami KI, Ohki I, Nagano M, Furuita F, Sugiki T, Kawano Y, Kawasaki T, Fujiwara T, Nakagawa A, Shimamoto K, Kojima C
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Journal Title
The Journal of Biological Chemistry
Volume: 289
Issue: 41
Pages: 28569-78
DOI
Description
(in press)(印刷中)
Related Report
Peer Reviewed
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