Single-molecule imaging analysis of oligomerization of GPCRs
| Project/Area Number |
26840055
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biophysics
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Yanagawa Masataka 国立研究開発法人理化学研究所, 佐甲細胞情報研究室, 研究員 (70609792)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | GPCR / 代謝型グルタミン酸受容体 / セロトニン受容体 / 1分子イメージング / 多量体化 / FRET / 生物物理学 / 薬理学 / 生化学 / 統合失調症 |
|---|
| Outline of Final Research Achievements |
G protein-coupled receptors (GPCRs) are major drug targets. It has been reported that various GPCRs form dimers regulating their functions. However, a role of higher-order oligomerization of GPCRs in living cell is unclear. Here, we performed single-molecule imaging and FRET analysis of metabotropic glutamate receptor (mGluR) and serotonine receptor (5HTR) as model GPCRs. Single-molecule imaging analysis in living cell demonstrated that the fraction of the immobile higher-order oligomer of mGluRs, which is related to the recruitment of receptors into clathrin-coated pits, increased upon activation. The FRET analysis suggested that a dissociation of mGluR/5HTR hetero-oligomer occurs upon activation of mGluR.
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Report
(4 results)
Research Products
(23 results)
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[Journal Article] Adaptation of cone pigments found in green rods for scotopic vision through a single amino acid mutation2017
Author(s)
Keiichi Kojima , Yuki Matsutani , Takahiro Yamashita , Masataka Yanagawa , Yasushi Imamoto , Yumiko Yamano , Akimori Wada , Osamu Hisatomi , Kanto Nishikawa , Keisuke Sakurai , Yoshinori Shichida
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Journal Title
Proceedings of the National Academy of Sciences
Volume: 印刷中
Related Report
Peer Reviewed
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[Journal Article] Origin of the low thermal isomerization rate of rhodopsin chromophore2015
Author(s)
Yanagawa, M.; Kojima, K.; Yamashita, T.; Iwamoto, Y.; Matsuyama, T.; Nakanishi, K.; Yamano, Y.; Wada, A.; Sako, Y.; Shichida, Y.
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Journal Title
Sci. Rep.
Volume: 5
Issue: 1
Pages: 11081-11081
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] Single-molecule pharmacology of a GPCR2017
Author(s)
Masataka Yanagawa, Michio Hiroshima, Yuichi Togashi, Takahiro Yamashita, Yoshinori Shichida, Masahiro Ueda, Masayuki Murata, Yasushi Sako
Organizer
University of Strasbourg-RIKEN Workshop on Membrane Lipidology
Place of Presentation
Region Grand Est (France)
Year and Date
2017-03-09
Related Report
Int'l Joint Research
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[Presentation] Molecular mechanism of the low thermal activation rate of visual pigments2015
Author(s)
Keiichi Kojima, Masataka Yanagawa, Takahiro Yamashita, Yuki Matsutani, Yasushi Imamoto, Take Matsuyama, Koji Nakanishi, Yumiko Yamano, Akimori Wada, Yasushi Sako, Yoshinori Shichida
Organizer
日本生物物理学会第53回年会
Place of Presentation
金沢大学(石川県金沢市)
Year and Date
2015-09-13
Related Report
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