Spatiotemporal visualization of intracellular calcium signal for regulation of sperm function
Project/Area Number |
26840061
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Cell biology
|
Research Institution | University of Tsukuba |
Principal Investigator |
SHIBA Kogiku 筑波大学, 生命環境系, 助教 (70533561)
|
Project Period (FY) |
2014-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
|
Keywords | 鞭毛運動 / 精子 / カルシウム |
Outline of Final Research Achievements |
This research was carried out to elucidate sperm function in the various stages of fertilization. Especially intracellular calcium signaling was focused. The following results were obtained. (1) To visualize sperm function spatiotemporally the system for sperm stimulation by using caged compounds were constructed. (2) Experiments using caged ATP revealed that calaxin is involved in propagation and switching of flagellar waveforms. (3) Soluble adenylyl cyclase critically plays a role in the regulation of flagellar motility and Ca2+-dependent regulation of flagellar waveform during chemotactic movement.
|
Report
(3 results)
Research Products
(36 results)
-
-
-
-
[Journal Article] Lactic acid is a sperm motility inactivation factor in the sperm storage tubules.2015
Author(s)
Matsuzaki M, Mizushima S, Hiyama G, Hirohashi N, Shiba K, Inaba K, Suzuki T, Dohra H, Ohnishi T, Sato Y, Kohsaka T, Ichikawa Y, Atsumi Y, Yoshimura T, Sasanami T.
-
Journal Title
Sci Rep.
Volume: 5
Issue: 1
Pages: 17643-17643
DOI
NAID
Related Report
Peer Reviewed / Open Access
-
[Journal Article] Chemotactic response with a constant delay-time mechanism in Ciona spermatozoa revealed by a high time resolution analysis of flagellar motility.2015
Author(s)
Miyashiro, D. Shiba, K., Miyashita, T., Baba, S., Yoshida, M., and Kamimura, S.
-
Journal Title
Biology Open
Volume: 4
Issue: 2
Pages: 109-118
DOI
Related Report
Peer Reviewed / Open Access
-
-
[Journal Article] Species and gamete-specific fertilization success of two sea urchins under near future levels of pCO2.2014
Author(s)
Sung CG, Kim TW, Park YG, Kang SG, Inaba K, Shiba K, Choi TS, Moon SD, Litvin S, Lee KT, Lee JS.
-
Journal Title
Journal of Marine Systems.
Volume: 137
Pages: 67-73
DOI
Related Report
Peer Reviewed
-
[Journal Article] The birth of quail chicks after intracytoplasmic sperm injection2014
Author(s)
Mizushima, S., Hiyama, G., Shiba, K., Inaba, K., Dohra, H., Ono, T., Shimada, K. and Sasanami, T.
-
Journal Title
Development
Volume: 141
Issue: 19
Pages: 3799-3806
DOI
NAID
Related Report
Peer Reviewed / Open Access
-
[Presentation] Sperm calcineurin is necessary for midpiece flexibility and male fertility2016
Author(s)
Haruhiko Miyata, Yuhkoh Satouh, Daisuke Mashiko, Masanaga Muto, Kaori Nozawa, Kogiku Shiba, Yoshitaka Fujihara, Ayako Isotani, Kazuo Inaba, Masahito Ikawa
Organizer
Biophysical Society 60th Annual Meeting
Place of Presentation
Los Angeles Convention Center, Los Angeles, CA, USA.
Year and Date
2016-02-27
Related Report
Int'l Joint Research
-
[Presentation] Sperm-specific calcineurin is necessary for midpiece flexibility and male fertility2016
Author(s)
Haruhiko Miyata, Yuhkoh Satouh, Daisuke Mashiko, Masanaga Muto, Kaori Nozawa, Kogiku Shiba, Yoshitaka Fujihara, Ayako Isotani, Kazuo Inaba, Masahito Ikawa
Organizer
国際シンポジウム "生殖細胞のエピゲノムダイナミクスとその制御"
Place of Presentation
京都大学百周年時計台記念館, 京都府、京都市
Year and Date
2016-02-17
Related Report
Int'l Joint Research
-
-
-
-
-
-
[Presentation] カタユウレイボヤ精子鞭毛における内腕ダイニンのキャラクタリゼーション2015
Author(s)
Osamu Kutomi, Katsutoshi Mizuno, Keiko Hirose, Lixy Yamada, Hitoshi Sawada, Kogiku Shiba, Kazuo Inaba
Organizer
第53回日本生物物理学会年会
Place of Presentation
金沢大学角間キャンパス, 石川県、金沢市
Year and Date
2015-09-13
Related Report
-
[Presentation] Inhibition of sperm-specific calcineurin for male contraception2015
Author(s)
Haruhiko Miyata, Yuhkoh Satouh, Daisuke Mashiko, Masanaga Muto, Kaori Nozawa, Kogiku Shiba, Yoshitaka Fujihara, Ayako Isotani, Kazuo Inaba, Masahito Ikawa
Organizer
Gordon Research Conference: Fertilization and the Activation of Development
Place of Presentation
Holderness, NH, USA.
Year and Date
2015-07-19
Related Report
Int'l Joint Research
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-