Spatiotemporal visualization of intracellular calcium signal for regulation of sperm function

2015 Fiscal Year Final Research Report

• Project/Area Number: 26840061
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Cell biology
• Research Institution: University of Tsukuba
• Principal Investigator: SHIBA Kogiku 筑波大学, 生命環境系, 助教 (70533561)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 鞭毛運動 / 精子 / カルシウム

Outline of Final Research Achievements

This research was carried out to elucidate sperm function in the various stages of fertilization. Especially intracellular calcium signaling was focused. The following results were obtained. (1) To visualize sperm function spatiotemporally the system for sperm stimulation by using caged compounds were constructed. (2) Experiments using caged ATP revealed that calaxin is involved in propagation and switching of flagellar waveforms. (3) Soluble adenylyl cyclase critically plays a role in the regulation of flagellar motility and Ca2+-dependent regulation of flagellar waveform during chemotactic movement.

Free Research Field

細胞生物学