Mapping neuropeptide neurons in the brain of Drosophila

• Project/Area Number: 26840110
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Animal physiology/Animal behavior
• Research Institution: Tohoku University
• Principal Investigator: Yamagata Nobuhiro 東北大学, 生命科学研究科, 助教 (50716248)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 神経ペプチド / コネクトミクス

Outline of Final Research Achievements

Neuropeptides play a key role in regulation of variety of animal behaviors including feeding and learning. To understand anatomical logic behind, we reconstructed in silico brain-wide neuropeptide map in Drosophila melanogaster. To this end, we developed a software pipeline for high-throughput brain registration processing. Combining this with GAL4/UAS gene expression system and confocal microscopy allowed me to visualize and map brain expression of all neuropeptide-specific GAL4s in the standardized brain coordinates, revealing brain neuropeptide ‘hot spots’ as well as inhibitory regulation of rewarding dopamine system by Allatostatin A.

Research Products

• [Journal Article] Four Individually Identified Paired Dopamine Neurons Signal Reward in Larval Drosophila. (2016)
  • Author(s): Rohwedder A, Wenz NL, Stehle B, Huser A, Yamagata N, Zlatic M, Truman JW, Tanimoto H, Saumweber T, Gerber B, Thum AS
  • Journal Title: Current Biology Volume: 26(5) Issue: 5 Pages: 661-669
  • DOI: 10.1016/j.cub.2016.01.012
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Reward signal in a recurrent circuit drives appetitive long-term memory formation. (2015)
  • Author(s): Ichinose T, Aso Y, Yamagata N, Abe A, Rubin GM, Tanimoto H.
  • Journal Title: eLife Volume: 4 Pages: 1-18
  • DOI: 10.7554/elife.10719
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Distinct dopamine neurons mediate reward signals for short- and long-term memories (2015)
  • Author(s): Yamagata N, Ichinose T, Aso Y, Placais P, Friedrich AB, Sima RJ, Preat T, Rubin GM, Tanimoto H
  • Journal Title: PNAS Volume: 112 Issue: 2 Pages: 578-583
  • DOI: 10.1073/pnas.1421930112
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Mushroom body output neurons encode valence and guide memory-based action selection in Drosophila (2014)
  • Author(s): Aso Y, Sitaraman D, Ichinose T, Kaun KR, Vogt K, Belliart-Guerin G, Placais PY, Robie AA, Yamagata N, Schnaitmann C, Rowell WJ, Johnston RM, Ngo TT, Chen N, Korff W, Nitabach MN, Heberlein U, Preat T, Branson KM, Tanimoto H, Rubin GM
  • Journal Title: Elife Volume: 4
  • DOI: 10.7554/elife.04580
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Converging circuits mediate temperature and shock aversive olfactory conditioning in Drosophila. (2014)
  • Author(s): Galili DS, Dylla KV, Ludke A, Friedrich AB, Yamagata N, Wong JY, Ho CH, Szyszka P, Tanimoto H.
  • Journal Title: Curr Biol. Volume: Aug 4;24(15) Issue: 15 Pages: 1712-1722
  • DOI: 10.1016/j.cub.2014.06.062
  • Peer Reviewed / Open Access
• [Presentation] Suppression of dopamine neurons mediates reward (2015)
  • Author(s): Nobuhiro Yamagata, Makoto Hiroi, Shu Kondo, Ayako Abe, Tetsuya Tabata, Hiromu Tanimoto
  • Organizer: NEUROBIOLOGY OF DROSOPHILA
  • Place of Presentation: Cold Spring Harbor Laboratory, USA
  • Year and Date: 2015-09-29
  • Int'l Joint Research
• [Presentation] ドーパミン神経抑制による記憶形成 (2015)
  • Author(s): 山方 恒宏
  • Organizer: 日本遺伝学会第87回大会
  • Place of Presentation: 東北大学川内北キャンパス（宮城県仙台市）
  • Year and Date: 2015-09-24
  • Invited
• [Presentation] 短期・長期記憶を誘導する異なるドーパミン細胞群 (2014)
  • Author(s): 山方 恒宏
  • Organizer: 日本動物学会 第85回 仙台大会
  • Place of Presentation: 仙台
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] Neural circuit for reward learning in the brain of the fruit fly, Drosophila melanogaster (2014)
  • Author(s): 山方 恒宏
  • Organizer: The 11th Japanese Drosophila Research Conference (JDRC11)
  • Place of Presentation: 金沢
  • Year and Date: 2014-06-04 – 2014-06-06