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Analysis of direct and indirect regulation of kisspeptin neurons on isotocin/vasotocin neurons

Research Project

Project/Area Number 26840111
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Animal physiology/Animal behavior
Research InstitutionThe University of Tokyo

Principal Investigator

Kanda Shinji  東京大学, 大学院理学系研究科(理学部), 准教授 (50634284)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsキスペプチン / バソトシン / GnRH / Ca2+イメージング / トランスジェニック / 神経ペプチド / 免疫組織化学 / メダカ / イソトシン / カルシウムイメージング
Outline of Final Research Achievements

In this study, we used physiological and histological approaches to examine the regulation of kisspeptin neural system on vasotonergic neural system in medaka. First, Ca2+ imaging experiments demonstrated that vasotocin neurons increase their intracellular calcium level in response to synthetic Kiss2 peptide. Moreover, by using a newly generated Kiss2 specific antiserum and a transgenic lines whose vasotocin neurons are labeled by GFP, close apposition of Kiss2 and vasotocin fibers was demonstrated. These lines of evidence strongly suggest that Kiss2 neurons regulate vasotocin neuronal activities.
In addition to these neuroanatomical approaches, we established knockout medaka for kisspeptin or vasotocin related genes. RNA seq analysis of their brains suggested some candidate neurotransmitters that can be regulated by kisspeptin or vasotocin neural systems in the brain. The analysis of these neuronal systems, which may be related to kisspeptin and/or vasotocin system, is underway.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (32 results)

All 2016 2015 2014 Other

All Journal Article (3 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 3 results,  Acknowledgement Compliant: 1 results) Presentation (26 results) (of which Int'l Joint Research: 10 results,  Invited: 6 results) Remarks (3 results)

  • [Journal Article] Evolution of the hypothalamic-pituitary-gonadal axis regulation in vertebrates revealed by knockout medaka.2016

    • Author(s)
      Takahashi, A., Kanda, S., Abe, T., and Oka, Y.
    • Journal Title

      Endocrinology

      Volume: 157 Issue: 10 Pages: 3994-4002

    • DOI

      10.1210/en.2016-1356

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Female specific glucose-sensitivity of GnRH1 neurons leads to sexually dimorphic inhibition of reproduction in medaka.2016

    • Author(s)
      Hasebe, M., Kanda, S., and Oka, Y.
    • Journal Title

      Endocrinology

      Volume: 157 Issue: 11 Pages: 4318-4329

    • DOI

      10.1210/en.2016-1352

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Kiss1 neurons drastically change their firing activity in accordance with the reproductive state: insights from a seasonal breeder.2014

    • Author(s)
      Hasebe M, Kanda S, Shimada H, Akazome Y, Abe H, Oka Y.
    • Journal Title

      Endocrinology

      Volume: 155 Issue: 12 Pages: 4868-4880

    • DOI

      10.1210/en.2014-1472

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Analysis of estrogen feedback mechanism of reproductive regulation in teleosts by using estrogen receprtor knockout female medaka2016

    • Author(s)
      Kayo, D., Kanda, S., Zempo, B., Oka, Y.
    • Organizer
      The joint meeting of the 22nd International Congress of Zoology and the 87th Meeting of the Zoological Society
    • Place of Presentation
      Okinawa Institute of Science and Technology Graduate University, Onna-son, Okinawa
    • Year and Date
      2016-11-14
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The kisspeptin neuronal system regulates vasotocin/isotocin neurons via release of neuropeptide B from vPOA Gpr54-1-expressing neurons2016

    • Author(s)
      Nakajo, M., Kanda, S., Karigo, T., and Oka, Y.
    • Organizer
      The joint meeting of the 22nd International Congress of Zoology and the 87th Meeting of the Zoological Society
    • Place of Presentation
      Okinawa Institute of Science and Technology Graduate University, Onna-son, Okinawa
    • Year and Date
      2016-11-14
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Electrophysiological and genetic demonstration that VT neuron is critical for water excretion in hypoosmotic conditions in medaka2016

    • Author(s)
      Shinya, M., Kanda, S., Takahashi, A., Konno, N., Hyodo, S., Kagawa, N., and Oka, Y.
    • Organizer
      The joint meeting of the 22nd International Congress of Zoology and the 87th Meeting of the Zoological Society
    • Place of Presentation
      Okinawa Institute of Science and Technology Graduate University, Onna-son, Okinawa
    • Year and Date
      2016-11-14
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Kisspeptin may be one of the evolutionary choices specific to mammals for the promotion of folliculogenesis2016

    • Author(s)
      Kanda, S., Arai, Y., Takahashi, A., Abe, T., and Oka, Y.
    • Organizer
      The joint meeting of the 22nd International Congress of Zoology and the 87th Meeting of the Zoological Society
    • Place of Presentation
      Okinawa Institute of Science and Technology Graduate University, Onna-son, Okinawa
    • Year and Date
      2016-11-14
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Characiform GnRH neuronal systems provides a good model for functional compensation by paralogous genes2016

    • Author(s)
      Ishida, M., Yang, C., Akazome, Y., Oka, Y., Kanda, S.
    • Organizer
      The joint meeting of the 22nd International Congress of Zoology and the 87th Meeting of the Zoological Society
    • Place of Presentation
      Okinawa Institute of Science and Technology Graduate University, Onna-son, Okinawa
    • Year and Date
      2016-11-14
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] GnRH receptor in LH cell is a critical red/green signal for LH surge2016

    • Author(s)
      Kanda, S., Arai, Y., Takahashi, A., Abe, T., and Oka, Y.
    • Organizer
      The 8th International Symposium on Fish Endocrinology
    • Place of Presentation
      Lindholmen Conference Centre, Gothenburg, Sweden
    • Year and Date
      2016-06-28
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Sex steroid-sensitive kisspeptin regulation of various endocrine systems2016

    • Author(s)
      Kanda, S., Nakajo, M., Karigo, T., Oka, Y.
    • Organizer
      The 8th Congress of the Asia and Oceania Society for Comparative Endocrinology (AOSCE)
    • Place of Presentation
      Korea University College of Medicine, Seoul, Korea
    • Year and Date
      2016-06-20
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Female specific glucose-sensing of GnRH neurons may cause the sexually dimorphic reproductive regulation in malnutritional states2016

    • Author(s)
      Hasebe, M., Kanda, S., Oka, Y.
    • Organizer
      The 8th Congress of the Asia and Oceania Society for Comparative Endocrinology (AOSCE)
    • Place of Presentation
      Korea University College of Medicine, Seoul, Korea
    • Year and Date
      2016-06-20
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] fshbおよびlhbそれぞれのノックアウトメダカを用いた真骨魚類生殖制御機構の解析2016

    • Author(s)
      安部智裕, 高橋晶子, 神田真司, 岡良隆
    • Organizer
      日本動物学会 第68回関東支部大会
    • Place of Presentation
      神奈川大学(神奈川県・横浜市)
    • Year and Date
      2016-03-12
    • Related Report
      2015 Research-status Report
  • [Presentation] メダカの特徴を活かした脊椎動物の生殖中枢制御機構の解析.in サントリー生有研シンポジウム「性と成熟:その普遍性と多様性を支える機構」2016

    • Author(s)
      神田真司
    • Organizer
      サントリー生有研シンポジウム「性と成熟:その普遍性と多様性を支える機構」
    • Place of Presentation
      サントリー生命科学財団(京都府・相楽郡)
    • Year and Date
      2016-02-05
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] Making the most of a small fish brain -elucidation of neuropeptide functions and neuronal network in medaka-2015

    • Author(s)
      Kanda, S.
    • Organizer
      日本分子生物学会 【ワークショップ】 小型魚類解体新書
    • Place of Presentation
      神戸ポートピアホテル(兵庫県・神戸市)
    • Year and Date
      2015-12-02
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] Changes in GnRH sensitivity of LH cells play a key role in seasonal regulation of reproduction2015

    • Author(s)
      Arai, Y., S. Kanda, T. Karigo, M. Hasebe, and Y. Oka.
    • Organizer
      Society for Neuroscience
    • Place of Presentation
      シカゴ (米国)
    • Year and Date
      2015-10-19
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Glucose-sensitivity of GnRH1 neurons cause down-regulation of GnRH1 neuronal activity by fasting, leading to the inhibition of the HPG axis.2015

    • Author(s)
      Hasebe, M., S. Kanda, and Y. Oka.
    • Organizer
      Society for Neuroscience
    • Place of Presentation
      シカゴ (米国)
    • Year and Date
      2015-10-19
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] ペプチドニューロンをモデルにして脊椎動物神経系機能の進化を理解する2015

    • Author(s)
      神田真司, 長谷部政治, 高橋晶子, 中城光琴, 苅郷友美, 鹿野悠, 小林由佳, 岡良隆
    • Organizer
      日本動物学会86回大会シンポジウム
    • Place of Presentation
      朱鷺メッセ(新潟県・新潟市)
    • Year and Date
      2015-09-18
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] HPG軸による生殖中枢制御に絶食が及ぼす影響の神経生物学的解析2015

    • Author(s)
      長谷部政治, 神田真司, 岡良隆
    • Organizer
      日本動物学会 第86回大会
    • Place of Presentation
      朱鷺メッセ(新潟県・新潟市)
    • Year and Date
      2015-09-17
    • Related Report
      2015 Research-status Report
  • [Presentation] 脳下垂体制御におけるキスペプチン神経系の新規機能2015

    • Author(s)
      中城光琴, 苅郷友美, 神田真司, 岡良隆
    • Organizer
      日本動物学会 第86回大会
    • Place of Presentation
      朱鷺メッセ(新潟県・新潟市)
    • Year and Date
      2015-09-17
    • Related Report
      2015 Research-status Report
  • [Presentation] メダカを用いたエストロジェンフィードバック機構の解析2015

    • Author(s)
      荒井勇樹, 神田真司, 苅郷友美, 長谷部政治, 岡良隆
    • Organizer
      日本動物学会 第86回大会
    • Place of Presentation
      朱鷺メッセ(新潟県・新潟市)
    • Year and Date
      2015-09-17
    • Related Report
      2015 Research-status Report
  • [Presentation] 遺伝子ノックアウト時のパラログ遺伝子による補償機能の解明2015

    • Author(s)
      神田真司
    • Organizer
      ユニークな少数派実験動物を扱う若手が 最先端アプローチを勉強する会
    • Place of Presentation
      岡崎カンファレンスセンター(愛知県・岡崎市)
    • Year and Date
      2015-08-18
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] Kisspeptin increases the spontaneous firing rate of vasotocin (VT) neurons in the brain of medaka2014

    • Author(s)
      Shikano Y, Kanda S, Oka Y.
    • Organizer
      Society for Neuroscience
    • Place of Presentation
      Washington, DC
    • Year and Date
      2014-11-15
    • Related Report
      2014 Research-status Report
  • [Presentation] Electrophysiological analyses of sex steroid-sensitive Kiss1 neurons in a seasonal breeder, medaka2014

    • Author(s)
      Hasebe M, Kanda S, Shimada H, Akazome Y, Oka Y.
    • Organizer
      Society for Neuroscience
    • Place of Presentation
      Washington, DC
    • Year and Date
      2014-11-15
    • Related Report
      2014 Research-status Report
  • [Presentation] Multidisciplinary analyses of gpr54-EGFP transgenic medaka reveal novel functions of the kisspeptin neuronal system.2014

    • Author(s)
      Nakajo M, Karigo T, Kanda S, Oka Y.
    • Organizer
      Society for Neuroscience
    • Place of Presentation
      Washington, DC
    • Year and Date
      2014-11-15
    • Related Report
      2014 Research-status Report
  • [Presentation] Functional divergence and evolution of steroid-sensitive kiss1/2 neuronal systems in vertebrates: amphibian as a model for understanding evolution of paralogous gene functions.2014

    • Author(s)
      Kanda S, Oka Y.
    • Organizer
      The 8th International Symposium on Amphibian and Reptilian Endocrinology and Neurobiology
    • Place of Presentation
      Okazaki, Japan
    • Year and Date
      2014-11-07
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] メダカ・バソトシンニューロンの自発発火活動に対するキスペプチンの効果の生理学的解析2014

    • Author(s)
      鹿野悠, 神田真司, 加川尚, 岡良隆.
    • Organizer
      日本動物学会第85回大会
    • Place of Presentation
      仙台
    • Year and Date
      2014-09-11
    • Related Report
      2014 Research-status Report
  • [Presentation] メダカ脳におけるエストロジェン受容体α発現ニューロンの可視化および神経線維投射の形態学的解析.2014

    • Author(s)
      善方文太郎, 神田真司, 赤染康久, 岡良隆
    • Organizer
      日本動物学会第85回大会
    • Place of Presentation
      仙台
    • Year and Date
      2014-09-11
    • Related Report
      2014 Research-status Report
  • [Presentation] キスペプチン神経系の摂食・内分泌系に対する制御.2014

    • Author(s)
      中城光琴, 苅郷友美, 神田真司, 岡良隆
    • Organizer
      日本動物学会第85回大会
    • Place of Presentation
      仙台
    • Year and Date
      2014-09-11
    • Related Report
      2014 Research-status Report
  • [Presentation] The input/output systems of kisspeptin neurons suggested by studies using transgenic medaka.2014

    • Author(s)
      Kanda S, Hasebe M, Nakajo M, Shikano Y, Takahashi A, Karigo T, Akazome Y, Oka Y.
    • Organizer
      27th Conference of European Comparative Endocrinologists
    • Place of Presentation
      Rennes, France
    • Year and Date
      2014-08-29
    • Related Report
      2014 Research-status Report
    • Invited
  • [Remarks] 神田真司ホームページ

    • URL

      http://www.biol.s.u-tokyo.ac.jp/users/naibunpi/kanda/

    • Related Report
      2015 Research-status Report
  • [Remarks] 神経回路の比較が教えてくれること 神田真司

    • URL

      http://www.biol.s.u-tokyo.ac.jp/users/naibunpi/kanda/

    • Related Report
      2014 Research-status Report
  • [Remarks] 繁殖状態のセンサーとしてはたらく脳内ニューロンを発見

    • URL

      https://www.s.u-tokyo.ac.jp/ja/press/1292/

    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2019-12-27  

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