| Project/Area Number |
26840115
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Genetics/Chromosome dynamics
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| Research Institution | Iwate Medical University |
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Principal Investigator |
Hiruta Chizue 岩手医科大学, 教養教育センター, 助教 (20723018)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | モデル生物開発 / ゲノム編集 / 単為生殖 / ミジンコ / ノックアウト / TALEN / ノックイン / 発生段階表 |
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| Outline of Final Research Achievements |
In order to be able to reveal the molecular mechanism of the parthenogenetic oogenesis in the water flea Daphnia pulex, I conducted microinjection of TALEN mRNAs into early embryos and examined the possibility of using TALEN to edit the genome. As a result, I have succeeded the development of targeted gene disruption (knock-out) by using Platinum TALEN in D. pulex. Now, I am engaged on the development of knock-in system by using TAL-PITCh. Moreover, I described the developmental staging of both sexes during embryogenesis in D. pulex to use for its phenotype analysis of knock-in/out strains.
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