host-pathogen interactions via noncoding RNA

• Project/Area Number: 26850026
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plant protection science
• Research Institution: The University of Tokyo
• Principal Investigator: Maejima Kensaku 東京大学, 大学院農学生命科学研究科(農学部), 助教 (20726062)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: ファイトプラズマ / sRNA

Outline of Final Research Achievements

The development of RNA-seq technology suitable for phytoplasma revealed that a large number of transcripts are generated from the genomic region where ORF region and ORF do not exist. We also established a method to extract only phytoplasma RNA from phytoplasma-infected plants and comprehensively identify transcription start sites on the genome. The sites are also found in RNA transcribed from noncoding regions. Some putative promoter sequences commonly found in phytoplasmas. These data are the first findings and directly contribute to elucidation of expression control of phytoplasma genes and small RNAs.

Research Products

• [Journal Article] Functional characterization of the principal sigma factor RpoD of phytoplasmas via an in vitro transcription assay. (2015)
  • Author(s): Miura C., Komatsu K., Maejima K., Nijo T., Kitazawa Y., Tomomitsu T., Yusa A., Himeno M., Oshima K., Namba S.
  • Journal Title: Scientific Reports Volume: 5 Issue: 1 Pages: 11893-11893
  • DOI: 10.1038/srep11893
  • Peer Reviewed / Open Access
• [Journal Article] Recognition of floral homeotic MADS-domain transcription factors by a phytoplasmal effector, phyllogen, induces phyllody (2014)
  • Author(s): Maejima K., Iwai R., Himeno M., Komatsu K., Kitazawa Y., Fujita N., Ishikawa K., Fukuoka M., Minato N., Yamaji Y., Oshima K., Namba S
  • Journal Title: The Plant J Volume: 78 Issue: 4 Pages: 541-554
  • DOI: 10.1111/tpj.12495
  • Peer Reviewed / Open Access
• [Journal Article] Onion yellow phytoplasma P38 protein plays a role in adhesion to the hosts. (2014)
  • Author(s): Neriya Y., Maejima K., Nijo T., Tomomitsu T., Yusa A., Himeno M., Netsu O., Hamamoto H., Oshima K., Namba S.
  • Journal Title: FEMS Microbiology Letters Volume: 361 Issue: 2 Pages: 115-122
  • DOI: 10.1111/1574-6968.12620
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The phytoplasmal virulence factor TENGU causes plant sterility by downregulating of the jasmonic acid and auxin pathways. (2014)
  • Author(s): Minato, N., Himeno, M., Hoshi, A., Maejima, K., Komatsu, K., Takebayashi, Y., Kasahara, H., Yusa, A., Yamaji, Y., Oshima, K., Kamiya, Y. & Namba, S.
  • Journal Title: Scientific reports Volume: 4 Issue: 1 Pages: 1-7
  • DOI: 10.1038/srep07399
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] ファイトプラズマの遺伝子発現制御のしくみをさぐる (2016)
  • Author(s): 前島健作, 三浦千裕, 岩渕望, 鯉沼宏章, 二條貴通, 北沢優悟, 煉谷裕太朗, 姫野未紗子, 大島研郎, 難波成任
  • Organizer: 日本マイコプラズマ学会
  • Place of Presentation: 長崎県医師会館（長崎県 長崎市）
  • Year and Date: 2016-06-24
  • Invited
• [Presentation] ファイトプラズマのシグマ因子RpoDを用いたin vitro転写系の確立 (2016)
  • Author(s): 三浦千裕・二條貴通・鯉沼宏章・岩渕望・姫野未紗子・前島健作・小松健・大島研郎・難波成任
  • Organizer: 平成28年度日本植物病理学会大会
  • Place of Presentation: 岡山コンベンションセンター
  • Year and Date: 2016-03-21
• [Presentation] ファイトプラズマのシグマ因子RpoDは多様な遺伝子の転写制御に関与する (2016)
  • Author(s): 二條貴通・三浦千裕・岩渕望・鯉沼宏章・北沢優悟・前島健作・小松健・大島研郎・難波成任
  • Organizer: 平成28年度日本植物病理学会大会
  • Place of Presentation: 岡山コンベンションセンター
  • Year and Date: 2016-03-21
• [Presentation] ナノ病原体の病原性シグナル (2014)
  • Author(s): 前島健作・難波成任
  • Organizer: 植物の中をめぐる多様なシグナル分子
  • Place of Presentation: 奈良先端科学技術大学院大学
  • Year and Date: 2014-11-18
  • Invited
• [Presentation] ファイトプラズマの病原性因子が引き起こす植物の形態変化 (2014)
  • Author(s): 前島健作・北沢優悟・吉田哲也・福岡美里・松田健太郎・煉谷裕太朗・姫野未紗子・大島研郎・難波成任
  • Organizer: 日本マイコプラズマ学会 第41回 学術集会
  • Place of Presentation: 東京大学
  • Year and Date: 2014-05-22 – 2014-05-23
  • Invited