Suppression of errors in reverse transcription
| Project/Area Number |
26850050
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied microbiology
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| Research Institution | Osaka Medical College |
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Principal Investigator |
Fukui Kenji 大阪医科大学, 医学部, 助教 (00466038)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 逆転写 / ミスマッチ塩基対 / DNA / RNA / 逆転写反応 / ミスマッチ / 高度好熱菌 / PCR / ミスマッチ認識タンパク質 |
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| Outline of Final Research Achievements |
Reverse transcription is essential for gene expression analysis, detection of pathogenic microorganisms, and other diagnostic technologies. In this study, we developed a novel method for suppressing non-specific amplifications during reverse transcription-related reactions. The previously-uncharacterized protein LLBP was isolated from Thermus thermophlus HB8 and its binding specificity towards DNA/RNA mismatch was confirmed. LLBP inhibited reverse transcription reaction when the primer-template complex contained a DNA/RNA mismatch. This protein would be utilized for improving the accuracy of reverse transcription-related technologies.
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Report
(3 results)
Research Products
(1 results)
