Suppression of errors in reverse transcription

Research Project

Project/Area Number	26850050
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Applied microbiology
Research Institution	Osaka Medical College
Principal Investigator	Fukui Kenji 大阪医科大学, 医学部, 助教 (00466038)
Project Period (FY)	2014-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000) Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords	逆転写 / ミスマッチ塩基対 / DNA / RNA / 逆転写反応 / ミスマッチ / 高度好熱菌 / PCR / ミスマッチ認識タンパク質
Outline of Final Research Achievements	Reverse transcription is essential for gene expression analysis, detection of pathogenic microorganisms, and other diagnostic technologies. In this study, we developed a novel method for suppressing non-specific amplifications during reverse transcription-related reactions. The previously-uncharacterized protein LLBP was isolated from Thermus thermophlus HB8 and its binding specificity towards DNA/RNA mismatch was confirmed. LLBP inhibited reverse transcription reaction when the primer-template complex contained a DNA/RNA mismatch. This protein would be utilized for improving the accuracy of reverse transcription-related technologies.