| Project/Area Number |
26850068
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bioorganic chemistry
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| Research Institution | Mie University (2015-2016)
Tohoku University (2014) |
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Principal Investigator |
MASUDA Yuichi 三重大学, 生物資源学研究科, 准教授 (90617755)
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| Co-Investigator(Renkei-kenkyūsha) |
KAI Kenji 大阪府立大学, 大学院生命環境科学研究科, 講師 (40508404)
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| Research Collaborator |
TANAKA Ren 東北大学, 大学院薬学研究科, 大学院生
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 環状ペプチド / カイコ / アポリポタンパク質B / X線結晶構造解析 / 核磁気共鳴 / 三次元構造 / 構造活性相関 / 分子プローブ / 有機化学 / 立体配座 / NMR / 光親和性標識 / 構造－活性相関 / 固相合成 / コンホメーション |
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| Outline of Final Research Achievements |
We achieved organic synthesis of PF1171 hexapeptides, cyclic peptides with paralytic activity against silkworms and inhibitory activity against production of apolipoprotein B. X-ray crystallography and nuclear magnetic resonance revealed significance of intramolecular hydrogen bonds in maintaining 3D structure of PF1171 hexapeptides. 3D structure-activity relationship study suggested that the 3D structure observed in the crystal is closely related to bioactivity. Based on the structure-activity relationship, we designed and synthesized molecular probes of PF1171 hexapeptides which can search for their target biomolecules.
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