Mechanisms of effects of gut microbiota on inflammation in the central nerve system

• Project/Area Number: 26850090
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Food science
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Miyauchi Eiji 国立研究開発法人理化学研究所, 統合生命医科学研究センター, 訪問研究員 (60634706)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 腸内細菌 / 多発性硬化症 / Th17 / EAE

Outline of Final Research Achievements

We investigated the gut microbes which are involved in the progression of inflammation in central nerve system using Experimental allergic encephalomyelitis (EAE) mice, animal model of multiple sclerosis. It has shown that Erysipelotrichaceae strain was significantly decreased in the mice with reduced EAE susceptibility. So we isolated the Erysipelotrichaceae strain from mice, and inoculated to germ free mice. The mice colonized with only Erysipelotrichaceae strain showed increased EAE severity compared to germ free mice, meaning that this bacterium have pathogenic roles in EAE. Further, we found that this bacterium forms biofilm and attaches to epithelial cells in the small intestine, and this might lead to the increased pathogenicity of antigen-specific T cells.

Research Products

• [Journal Article] Plasmodium berghei ANKA causes intestinal malaria associated with dysbiosis (2015)
  • Author(s): Taniguchi T, Miyauchi E, Nakamura S, Hirai M, Suzue K, Imai T, Nomura T, Handa T, Okada H, Shimokawa C, Onishi R, Olia A, Hirata J, Tomita H, Ohno H, Horii T, Hisaeda H
  • Journal Title: Scientific Reports Volume: 5:15699 Issue: 1 Pages: 1-12
  • DOI: 10.1038/srep15699
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] フラクトオリゴ糖による腸内細菌叢修飾のBiogeographic解析 (2016)
  • Author(s): 宮内栄治、大野博司
  • Organizer: 日本農芸化学会2016年度大会
  • Place of Presentation: 札幌コンベンションセンター・札幌市・北海道
  • Year and Date: 2016-03-28
• [Presentation] The role of the gut microbiota in the development of experimental autoimmune encephalomyelitis (2015)
  • Author(s): MIYAUCHI Eiji, KIM Seok-Won, OHNO Hiroshi
  • Organizer: 第44回日本免疫学会学術集会
  • Place of Presentation: 札幌コンベンションセンター・札幌市・北海道
  • Year and Date: 2015-11-20
• [Presentation] High-affinity, poly-reactive IgA is required for gut homeostatic maintenance to prevent colitis in mice (2015)
  • Author(s): OKAI Shinsaku, USUI Fumihito, HASEGAWA Makoto, NAKAMURA Toshinobu, YAMAMOTO Kazuya, NISHIYAMA Eri, MORI Hiroshi, YAMADA Takuji, KUROKAWA Ken, KATO Tamotsu, MIYAUCHI Eiji, OHNO Hiroshi, SHINKURA Reiko
  • Organizer: 第44回日本免疫学会学術集会
  • Place of Presentation: 札幌コンベンションセンター・札幌市・北海道
  • Year and Date: 2015-11-19
• [Presentation] 腸内細菌による中枢神経系炎症の制御 (2014)
  • Author(s): 宮内栄治
  • Organizer: HiHA 第３回Workshop
  • Place of Presentation: 広島大学
  • Year and Date: 2014-12-26
  • Invited
• [Presentation] 腸内細菌による中枢神経系炎症の制御 (2014)
  • Author(s): 宮内栄治、加藤完、川住雅美、新奈緒子、大野博司
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-11-28
• [Book] 医学のあゆみ（粘膜免疫Update; 腸内細菌による多発性硬化症の制御） (2015)
  • Author(s): 宮内栄治
  • Total Pages: 6
  • Publisher: 医歯薬出版株式会社
• [Book] 医学のあゆみ (2015)
  • Author(s): 宮内栄治
  • Total Pages: 6
  • Publisher: 医歯薬出版