Bioactivity of 8-hydroxyeicosapentaenoic acid (8-HEPE) and 8-HEPE metabolisms.
| Project/Area Number |
26850135
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Aquatic life science
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| Research Institution | Iwate Biotechnology Research Center |
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Principal Investigator |
Yamada Hidetoshi 公益財団法人岩手生物工学研究センター, その他部局等, 研究員 (70511955)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 8-HEPE / オキアミ / PPAR / 肥満 / 8-ヒドロキシエイコサペンタエン酸 / ペルオキシソーム増殖因子活性化受容体 |
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| Outline of Final Research Achievements |
We identified 8-Hydroxyeicosapentaenoic acid (8-HEPE) as new PPAR ligand using with luciferase reporter assay from pacific krill. 8-HEPE has a greater affinity for PPAR activation than EPA; it is possible that 8-HEPE might be of value in the treatment of obesity and metabolic syndrome. In this study, we examined 1) 8-HEPE content of marine products, 2) metabolism of 8-HEPE in human and mouse cells, 3) bioactivity of 8-HEPE via PPAR activation in vitro and in vivo.
Our results indicated that 8-HEPE was a characteristic compound of the crustacea. And we demonstrated that 8-HEPE induced fatty acid beta-oxidation, adipogenesis and glucose uptake in vitro. Furthermore, the mice experiences, we demonstrated that 8-HEPE has a larger positive effect on metabolic syndrome than EPA and that 8-HEPE acts by inducing PPARalpha activation in the liver.
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Report
(3 results)
Research Products
(4 results)
