Study on molecular mechanism of influenza virus infections in chickens

• Project/Area Number: 26850178
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Veterinary medical science
• Research Institution: Hokkaido University
• Principal Investigator: Okamatsu Masatoshi 北海道大学, 獣医学研究科, 准教授 (00507163)
• Research Collaborator: NISHIHARA Syoko 創価大学, 工学部, 教授 ; TAKASE Sayaka 創価大学, 工学部, 教授
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: インフルエンザウイルス / レセプター / ニワトリ / ヘマグルチニン / 膜融合活性 / ノイラミニダーゼ / カモ / 硫酸化糖鎖 / 病原性 / 膜融合 / シアル酸糖鎖

Outline of Final Research Achievements

To reveal mechanism of intersperses transmission of avian influenza viruses between ducks, natural host, and chickens. We revealed that chicken influenza viruses preferentially bind to not only fucosylated but also sulfated alpha 2,3 sialoglycans as the receptor. These receptors were actually detected epithelial cells of chicken trachea, where virus replicate. Furthermore, membrane fusion activity of hemagglutinin protein between virus envelop and cell membrane are related to pathogenicity to chickens in some viruses, not all. Difference of neuraminidase activity of virus which derived from deletion of the stalk region also related to the pathogenicity to chickens. Our results indicate that several virus proteins are responsible for virus transmission and pathogenicity of avian influenza viruses to chickens.

Research Products

• [Int'l Joint Research] Depertment of animal health/Regional Animal Health Office No 7(ベトナム)
• [Int'l Joint Research] Utrecht University(オランダ)
• [Journal Article] A cross-sectional study to quantify the prevalence of avian influenza viruses in poultry at intervention and non-intervention live bird markets in central Vietnam, 2014. (2017)
  • Author(s): Chu DH, Stevenson MA, Nguyen LV, Isoda N, Firestone SM, Nguyen TN, Nguyen LT, Matsuno K, Okamatsu M, Kida H, Sakoda Y.
  • Journal Title: Transbound Emerg Dis. Volume: 印刷中 Issue: 6 Pages: 1991-1999
  • DOI: 10.1111/tbed.12605
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Characterization of Highly Pathogenic Avian Influenza Virus A(H5N6), Japan, November 2016. (2017)
  • Author(s): (8)Okamatsu, M., Ozawa, M., Soda, K., Takakuwa, H., Haga, A., Hiono, T., Matsuu, A., Uchida, Y., Iwata, R., Matsuno, K., Kuwahara, M., Yabuta, T., Usui, T., Ito, H., Onuma, M., Sakoda, Y., Saito, T., Otsuki, K., Ito, T., Kida, H.
  • Journal Title: Emerging Infectious Diseases Volume: 23(4) Issue: 4 Pages: 691-695
  • DOI: 10.3201/eid2304.161957
  • NAID: 120006337764
  • Peer Reviewed / Open Access
• [Journal Article] Antigenic diversity of H5 highly pathogenic avian influenza viruses of clade 2.3.4.4 isolated in Asia. (2017)
  • Author(s): (7)Ohkawara, A., Okamatsu, M., Ozawa, M., Chu, D.H., Nguyen, L.T., Hiono, T., Matsuno, K., Kida, H., Sakoda, Y.
  • Journal Title: Microbiology and Immunology Volume: 印刷中 Issue: 5 Pages: 149-158
  • DOI: 10.1111/1348-0421.12478
  • NAID: 120006466464
  • Peer Reviewed
• [Journal Article] Is the optimal pH for membrane fusion in host cells by avian influenza viruses related to host range and pathogenicity? (2016)
  • Author(s): Okamatsu M, Motohashi Y, Hiono T, Tamura T, Nagaya K, Matsuno K, Sakoda Y, Kida H.
  • Journal Title: Arch Virol. Volume: 161 Issue: 8 Pages: 2235-2242
  • DOI: 10.1007/s00705-016-2902-z
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Complete Genome Sequence of the Avian Paramyxovirus Serotype 5 Strain APMV-5/budgerigar/Japan/TI/75. (2016)
  • Author(s): Hiono T, Matsuno K, Tuchiya K, Lin Z, Okamatsu M, Sakoda Y.
  • Journal Title: Genome Announc. Volume: 4 Issue: 5
  • DOI: 10.1128/genomea.01005-16
  • Peer Reviewed
• [Journal Article] Protective efficacy of stockpiled vaccine against H5N8 highly pathogenic avian influenza virus isolated from a chicken in Kumamoto prefecture, Japan, in 2014 (2016)
  • Author(s): Gamoh K, Nakamizo M, Okamatsu M, Sakoda Y, Kida H, Suzuki S.
  • Journal Title: Journal of Veterinary Medical Science Volume: 78 Issue: 1 Pages: 139-142
  • DOI: 10.1292/jvms.15-0324
  • NAID: 130005121601
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access
• [Journal Article] Amino acid residues at positions 222 and 227 of the hemagglutinin together with the neuraminidase determine binding of H5 avian influenza viruses to sialyl Lewis X (2016)
  • Author(s): Hiono T, Okamatsu M, Igarashi M, McBride R, de Vries RP, Peng W, Paulson JC, Sakoda Y, Kida H
  • Journal Title: Arch Virol Volume: 161(2) Issue: 2 Pages: 307-316
  • DOI: 10.1007/s00705-015-2660-3
  • NAID: 120006661332
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Experimental infection of highly and low pathogenic avian influenza viruses to chickens, ducks, tree sparrows, jungle crows, and black rats for the evaluation of their roles in virus transmission (2016)
  • Author(s): Hiono T, Okamatsu M, Yamamoto N, Ogasawara K, Endo M, Kuribayashi S, Shichinohe S, Motohashi Y, Chu DH, Suzuki M, Ichikawa T, Nishi T, Abe Y, Matsuno K, Tanaka K, Tanigawa T, Kida H, Sakoda Y.
  • Journal Title: Vet Microbiol Volume: 182 Pages: 108-115
  • DOI: 10.1016/j.vetmic.2015.11.009
  • Peer Reviewed
• [Journal Article] Comparison of pathogenicities of H7 avian influenza viruses via intranasal and conjunctival inoculation in cynomolgus macaques (2016)
  • Author(s): Shichnohe S, Itoh Y, Nakayama M, Ozaki H, Soda K, Ishigaki H, Okamatsu M, Sakoda Y, Kida H, Ogasawara K
  • Journal Title: Virology Volume: 493 Pages: 31-38
  • DOI: 10.1016/j.virol.2016.03.007
  • Peer Reviewed
• [Journal Article] Is optimal pH for membrane fusion to host cells by avian influenza viruses related to host range and pathogenicity? (2016)
  • Author(s): Okamatsu M, Motohashi Y, Hiono T, Tamura T, Nagaya K, Matsuno K, Sakoda Y, Kida H.
  • Journal Title: Arch Virol Volume: 印刷中
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Fluorescent immunochromatography for rapid and sensitive typing of seasonal influenza viruses (2015)
  • Author(s): Sakurai A, Takayama K, Nomura N, Kajiwara N, Okamatsu M, Yamamoto N, Tamura T, Yamada J, Hashimoto M, Sakoda Y, Suda Y, Kobayashi Y, Kida H, Shibasaki F.
  • Journal Title: PLoS One Volume: 10 (2) Issue: 2 Pages: e0116715-e0116715
  • DOI: 10.1371/journal.pone.0116715
  • Peer Reviewed / Open Access
• [Journal Article] Genetic and antigenic characterization of H5 and H7 influenza viruses isolated from migratory water birds in Hokkaido, Japan and Mongolia from 2010 to 2014 (2015)
  • Author(s): Hiono T, Ohkawara A, Ogasawara K, Okamatsu M, Tamura T, Chu DH, Suzuki M, Kuribayashi S, Shichinohe S, Takada A, Ogawa H, Yoshida R, Miyamoto H, Nao N, Furuyama W, Maruyama J, Eguchi N, Ulziibat G, Enkhbold B, Shatar M, Jargalsaikhan T, Byambadorj S, Damdinjav B, Sakoda Y, Kida H.
  • Journal Title: Virus Genes Volume: 51 (1) Issue: 1 Pages: 57-68
  • DOI: 10.1007/s11262-015-1214-9
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] A Single Amino Acid in the M1 Protein Responsible for the Different Pathogenic Potentials of H5N1 Highly Pathogenic Avian Influenza Virus Strains (2015)
  • Author(s): Nao N, Kajihara M, Manzoor R, Maruyama J, Yoshida R, Muramatsu M, Miyamoto H, Igarashi M, Eguchi N, Sato M, Kondoh T, Okamatsu M, Sakoda Y, Kida H, Takada A
  • Journal Title: PLoS One Volume: 10(9) Issue: 9 Pages: e0137989-e0137989
  • DOI: 10.1371/journal.pone.0137989
  • NAID: 120005672370
  • Peer Reviewed / Open Access
• [Journal Article] The zoonotic potential of avian influenza viruses isolated from wild waterfowl in Zambia (2014)
  • Author(s): Simulundu E, Nao N, Yabe J, Muto NA, Sithebe T, Sawa H, Manzoor R, Kajihara M, Muramatsu M, Ishii A, Ogawa H, Mweene AS, Takada A
  • Journal Title: Arch. Virol. Volume: 159 Issue: 6 Pages: 2633-2640
  • DOI: 10.1371/journal.ppat.1004192
  • NAID: 120005656330
  • Peer Reviewed / Open Access
• [Journal Article] Potency of an inactivated influenza vaccine prepared from A/duck/Mongolia/119/2008 (H7N9) against the challenge with A/Anhui/1/2013 (H7N9). (2014)
  • Author(s): Chu DH, Sakoda Y, Nishi T, Hiono T, Shichinohe S, Okamatsu M, Kida H.
  • Journal Title: Vaccine Volume: 32 Issue: 28 Pages: 3473-9
  • DOI: 10.1016/j.vaccine.2014.04.060
  • Peer Reviewed
• [Journal Article] Potency of an inactivated influenza vaccine prepared from A/duck/Hong Kong/960/1980 (H6N2) against a challenge with A/duck/Vietnam/OIE-0033/2012 (H6N2) in mice. (2014)
  • Author(s): Nishi T, Sakoda Y, Okamatsu M, Kida H
  • Journal Title: Arch Virol. Volume: 159 Issue: 10 Pages: 2567-74
  • DOI: 10.1007/s00705-014-2107-2
  • NAID: 120005656329
  • Peer Reviewed
• [Journal Article] A chicken influenza virus recognizes fucosylated α2,3 sialoglycan receptors on the epithelial cells lining upper respiratory tracts of chickens. (2014)
  • Author(s): Hiono T, Okamatsu M, Nishihara S, Takase-Yoden S, Sakoda Y, Kida H.
  • Journal Title: Virology Volume: 456-457 Pages: 131-8
  • DOI: 10.1016/j.virol.2014.03.004
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] 近年分離されたH5ウイルスの抗原性と抗原変異株の出現メカニズムの解析 (2016)
  • Author(s): 大河原彩子、Lam Thanh Nguyen、岡松正敏、小澤真、 Huy Duc Chu、日尾野隆大、松野啓太、迫田義博
  • Organizer: 平成28年度鶏病研究会 北海道支部会 技術検討会
  • Place of Presentation: 北海道獣医師会館、札幌
• [Presentation] Evaluation of the roles of sparrows, jungle crows, and black rats in virus transmission of highly and low pathogenic avian influenza viruses (2015)
  • Author(s): Masatoshi Okamatsu, Takahiro Hiono, Kohei Ogasawara, Saya Kuribayashi, Shintaro Shichinohe, Duc-Huy Chu, Mizuho Suzuki, Takaya Ichikawa, Tatsuya Nishi, Keita Matsuno, Tsutomu Tanigawa, Kazuyuki Tanaka, Hiroshi Kida, Yoshihiro Sakoda
  • Organizer: 第63回 日本ウイルス学会学術集会
  • Place of Presentation: 福岡国際会議場、福岡市、福岡県
  • Year and Date: 2015-11-22
• [Presentation] 近年アジア地域で分離された鳥インフルエンザウイルスのアヒル、スズメ、カラス、およびクマネズミに対する病原性 (2015)
  • Author(s): 小笠原浩平,岡松正敏,日尾野隆大,七戸新太郎,栗林沙弥,市川貴也,西達也,Duc-Huy Chu,鈴木瑞穂,遠藤真由美,田中和之,谷川力,喜田宏,迫田義博
  • Organizer: 第158回 日本獣医学会学術集会
  • Place of Presentation: 北里大学獣医学部、十和田市、青森県
  • Year and Date: 2015-09-07
• [Presentation] The 220-loop of the hemagglutinin determines the recognition of fucosylated SAα2,3Gal glycans existing on the epithelial cells of chicken trachea (2015)
  • Author(s): T. Hiono, M. Okamatsu, M. Igarashi, R. MacBride, R. P de Vries, J. C. Paulson, Y. Sakoda, H. Kida
  • Organizer: 9th International Synposium on Avian influenza
  • Place of Presentation: UGA Hotel and Conference Center, Athens, GA, USA
  • Year and Date: 2015-04-12
  • Int'l Joint Research
• [Presentation] 糖鎖アレイを用いたカモとニワトリのインフルエンザウイルスのレセプター特異性 (2014)
  • Author(s): 岡松正敏, DE VRIES Robert, 日尾野隆大, 迫田義博, PAULSON C James, 喜田宏
  • Organizer: 日本獣医学会学術集会
  • Place of Presentation: 北海道大学
  • Year and Date: 2014-09-09 – 2014-09-12
• [Presentation] 糖鎖アレイを用いたカモとニワトリのインフルエンザウイルスのレセプター特異性 (2014)
  • Author(s): 岡松正敏, DE VRIES Robert, 日尾野隆大, 迫田義博, PAULSON C James, 喜田宏
  • Organizer: 日本糖質学会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2014-08-10 – 2014-08-12
• [Remarks] 北海道大学大学院獣医学研究科 微生物学教室
  • URL: http://www.vetmed.hokudai.ac.jp/organization/microbiol/index.html