Study on anti-viral innate immune responses induced by infection with bradyzoite stage of Toxoplasma gondii

• Project/Area Number: 26850183
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Veterinary medical science
• Research Institution: Kagoshima University
• Principal Investigator: Masatani Tatsunori 鹿児島大学, 農水産獣医学域獣医学系, 准教授 (70614072)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: トキソプラズマ / ウイルス / 自然免疫 / 共感染 / 病原体

Outline of Final Research Achievements

Toxoplasma gondii differentiates to bradyzoite (Bz) stage in host. We found that expression levels of some anti-viral proteins in Bz-infected cultured cells were higher than those in uninfected and Tz-infected cells. Aim of this study is to reveal the mechanism and importance of the phenomenon. We found that expression levels of mRNA of anti-viral proteins in brain of mice with chronic infection of T. gondii. We also found that mice with chronic infection of T. gondii have resistant to virus infections. STAT1, a transcription factor of innate immunity, was activated in human fibroblast with Bz stage of T. gondii. Taken together, our study revealed the fascinating effect of chronic infection of T. gondii to host.

Research Products

• [Journal Article] Seroprevalence of <i>Toxoplasma gondii</i> in free-ranging and feral cats on Amami Oshima Island, Japan (2017)
  • Author(s): MATSUU Aya、YOKOTA Shin-ichi、ITO Keiko、MASATANI Tatsunori
  • Journal Title: Journal of Veterinary Medical Science Volume: 79 Issue: 11 Pages: 1853-1856
  • DOI: 10.1292/jvms.17-0359
  • NAID: 130006206581
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed
• [Journal Article] トキソプラズマ症 (2017)
  • Author(s): 正谷達謄
  • Journal Title: 鹿児島県獣医師会会報 Volume: 59 Pages: 1-3
• [Journal Article] Detection and molecular characterization of Babesia, Theileria, and Hepatozoon species in hard ticks collected from Kagoshima, the southern region in Japan (2017)
  • Author(s): Masatani T, Hayashi K, Andoh M, Tateno M, Endo Y, Asada M, Kusakisako K, Tanaka T, Gokuden M, Hozumi N, Nakadohzono F, Matsuo T
  • Journal Title: Ticks and Tick Borne Diseases Volume: 印刷中 Issue: 4 Pages: 581-587
  • DOI: 10.1016/j.ttbdis.2017.03.007
  • Peer Reviewed / Open Access
• [Journal Article] トキソプラズマ症 (2017)
  • Author(s): 正谷達謄
  • Journal Title: 鹿児島県獣医師会雑誌 Volume: 印刷中
• [Journal Article] Dynamics of Theileria orientalis genotype population in cattle in a year-round grazing system. (2016)
  • Author(s): Masatani, T., Yoshihara, S., Matsubara, A., Gotoh, T., Takahashi, H., Tanaka, T., Andoh, M., Endo, Y. and Matsuo, T.
  • Journal Title: Acta Parasitol. Volume: 61 (2) Issue: 2 Pages: 419-424
  • DOI: 10.1515/ap-2016-0056
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Isolation and molecular characterization of porcine epidemic diarrhea viruses collected in Japan in 2014 (2016)
  • Author(s): Horie M, Kabemura M, Matasani T, Matsuu A, Ozawa M
  • Journal Title: Archives of Virology Volume: 161 Issue: 8 Pages: 2189-2195
  • DOI: 10.1007/s00705-016-2900-1
  • Peer Reviewed
• [Journal Article] Identification and functional analysis of a novel mitochondria-localized 2-Cys peroxiredoxin, BbTPx-2, from Babesia bovis (2016)
  • Author(s): Masatani T, Asada M, Hakimi H, Hayashi K, Yamagishi J, Kawazu S, Xuan X
  • Journal Title: Parasitology Research Volume: 115(8) Issue: 8 Pages: 3139-3145
  • DOI: 10.1007/s00436-016-5071-9
  • NAID: 120006390950
  • Peer Reviewed
• [Journal Article] Roles of the rabies virus phosphoprotein isoforms in pathogenesis (2016)
  • Author(s): Okada K, Ito N, Yamaoka S, Masatani T, Ebihara H, Nakagawa K, Mitake H, Okadera K, Sugiyama M
  • Journal Title: Journal of Virology Volume: 90 Issue: 18 Pages: 8226-8273
  • DOI: 10.1128/jvi.00809-16
  • Peer Reviewed
• [Journal Article] Molecular epidemiological study of adenovirus infecting western lowland gorillas and humans in and around Moukalaba-Doudou National Park (Gabon). (2016)
  • Author(s): Nze-Nkogue C, Horie M, Fujita S, Ogino M, Kobayashi Y, Mizukami K, Masatani T, Ezzikouri S, Matsuu A, Mizutani T, Ozawa M, Yamato O, Ngomanda A, Yamagiwa J, Tsukiyama-Kohara K
  • Journal Title: Virus Genes Volume: 52 Issue: 5 Pages: 671-678
  • DOI: 10.1007/s11262-016-1360-8
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Course of induced infection by Eimeria krijgsmannni in immunocompetent and immunodeficient mice (2016)
  • Author(s): Yuina Ono, Makoto Matsubayashi, Hiroaki Kawaguchi, Masashi Tsujio, Masanobu Mizuno, Tetsuya Tanaka, Tatsunori Masatani, Toshihiro Matsui, Tomohide Matsuo
  • Journal Title: Acta Parasitologica Volume: 115(1) Issue: 1 Pages: 211-5
  • DOI: 10.1007/s00436-015-4737-z
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Prevalence of anti-Toxoplasma gondii antibody in domestic horses in Japan (2016)
  • Author(s): Masatani T, Takashima Y, Takasu M, Matsuu A, Amaya T.
  • Journal Title: Parasitology International Volume: 65 Issue: 2 Pages: 146-150
  • DOI: 10.1016/j.parint.2015.11.006
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Contribution of the interaction between the rabies virus P protein and I-kappa B kinase e; to the inhibition of type I IFN induction signalling. (2016)
  • Author(s): Masatani T, Ozawa M, Yamada K, Ito N, Horie M, Matsuu A, Okuya K, Tsukiyama-Kohara K, Sugiyama M, Nishizono A.
  • Journal Title: J Gen Virol. Volume: 97 Issue: 2 Pages: 316-326
  • DOI: 10.1099/jgv.0.000362
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Genetic diversity of highly pathogenic H5N8 avian influenza viruses at a single overwintering site of migratory birds in Japan, 2014/15 (2015)
  • Author(s): Makoto Ozawa, Aya Matsuu, Kaori Tokorozaki, Masayuki Horie, Tatsunori Masatani, Hiroko Nakagawa, Kosuke Okuya, Toshiko Kawabata, Shigehisa Toda
  • Journal Title: Euro Surveillance Volume: 20 Issue: 20 Pages: 15-27
  • DOI: 10.2807/1560-7917.es2015.20.20.21132
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Genetic and serological surveillance for non-primate hepacivirus in horses in Japan (2015)
  • Author(s): Aya Matsuu, Seiji Hobo, Kunihide Ando, Takashi Sanekata, Fumio Sato, Yoshiro Endo, Tomohiko Amaya, Tomohiro Osaki, Masayuki Horie, Tatsunori Masatani, Makoto Ozawa, Kyoko Tsukiyama-Kohara
  • Journal Title: Veterinary Microbiology Volume: 179 Issue: 3-4 Pages: 219-227
  • DOI: 10.1016/j.vetmic.2015.05.028
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Molecular characterization of Babesia bovis M17 leucine aminopeptidase and inhibition of Babesia growth by bestatin (2015)
  • Author(s): Aboge GO, Cao S, Terkawi MA, Masatani T, Goo Y, AbouLaila M, Nishikawa Y, Igarashi I, Suzuki H, Xuan X.
  • Journal Title: Journal of Parasitology Volume: 101 Issue: 5 Pages: 536-541
  • DOI: 10.1645/15-745.1
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Molecular detection and characterization of Babesia bovis, Babesia bigemina, Theileria species and Anaplasma marginale isolated from cattle in Kenya. (2015)
  • Author(s): Adjou Moumouni PF, Aboge GO, Terkawi MA, Masatani T, Cao S, Kamyingkird K, Jirapattharasate C, Zhou M, Wang G, Liu M, Iguchi A, Vudriko P, Ybanez AP, Inokuma H, Shirafuji-Umemiya R, Suzuki H, Xuan X.
  • Journal Title: Parasites & Vectors Volume: 8 Issue: 1 Pages: 496-496
  • DOI: 10.1186/s13071-015-1106-9
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Functional analysis of recombinant 2-Cys peroxiredoxin from the hard tick Haemaphysalis longicornis (2015)
  • Author(s): Kusakisako K, Masatani T, Miyata T, Galay RL, Maeda H, Talactac MR, Tsuji N, Mochizuki M, Fujisaki K, Tanaka T.
  • Journal Title: Insect Molecular Biology Volume: 25 Issue: 1 Pages: 16-23
  • DOI: 10.1111/imb.12193
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] 原虫病学とウイルス学の融合的研究 (2015)
  • Author(s): 正谷達謄
  • Journal Title: 獣医寄生虫学会誌 Volume: 14 Pages: 31-38
• [Journal Article] Molecular characterization of Cryptosporidium parvum from two different Japanese prefectures, Okinawa and Hokkaido. (2014)
  • Author(s): Ichikawa-Seki M, Aita J, Masatani T, Suzuki M, Nitta Y, Tamayose G, Iso T, Suganuma K, Fujiwara T, Matsuyama K, Niikura T, Yokoyama N, Suzuki H, Yamakawa K, Inokuma H, Itagaki T, Zakimi S, Nishikawa Y.
  • Journal Title: Parasitol Int. Volume: 64 Issue: 2 Pages: 161-166
  • DOI: 10.1016/j.parint.2014.11.007
  • Peer Reviewed
• [Presentation] 宿主を操る寄生虫・トキソプラズマのお話 (2016)
  • Author(s): 正谷達謄
  • Organizer: 第9回ボルナウイルス研究会
  • Place of Presentation: 鹿児島大学郡元キャンパス（鹿児島県鹿児島市）
  • Year and Date: 2016-01-27
  • Invited
• [Presentation] Toxoplasma gondii K-3株のシスト形成能に関する研究 (2015)
  • Author(s): 小山田昇平、正谷達謄、辻尾祐志、田仲哲也、松井利博、松尾智英
  • Organizer: 第158回日本獣医学会学術集会
  • Place of Presentation: 北里大学十和田キャンパス（青森県十和田市）
  • Year and Date: 2015-09-07
• [Presentation] 細胞内寄生原虫トキソプラズマが誘導する自然免疫応答とその抗ウイルス効果 (2014)
  • Author(s): 正谷達謄、山岸潤也、玄学南
  • Organizer: 第62回日本ウイルス学会学術集会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2014-11-10 – 2014-11-12
• [Presentation] トキソプラズマ潜伏感染が誘導する宿主抗ウイルス自然免疫応答とその意義 (2014)
  • Author(s): 正谷達謄、山岸潤也、玄学南
  • Organizer: 第155回日本獣医学会学術集会
  • Place of Presentation: 北海道大学（北海道札幌市）
  • Year and Date: 2014-09-09 – 2014-09-12
  • Invited
• [Presentation] Anti-viral innate immune responses induced by infection with bradyzoitestage of Toxoplasma gondii (2014)
  • Author(s): Tatsunori Masatani, Junya Yamagishi, Xuenan Xuan
  • Organizer: 第13回国際寄生虫学病学会議（ICOPAXIII）
  • Place of Presentation: メキシコシティ、メキシコ
  • Year and Date: 2014-08-10 – 2014-08-15