Coevolution between Bartonella quintana and the natural reservoir and the pathogenicity of macaque-derived B. quintana for humans.

• Project/Area Number: 26850188
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Veterinary medical science
• Research Institution: Nihon University
• Principal Investigator: SATO Shingo 日本大学, 生物資源科学部, 助手 (60708593)
• Research Collaborator: MARUYAMA Soichi ; KABEYA Hidenori ; SUZUKI Kazuo ; TAMATE Hidetoshi ; YAMAZAKI Shouki ; CHOMEL Bruno B.
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: Bartonella quintana / ニホンザル / 塹壕熱 / MLST / 赤血球

Outline of Final Research Achievements

Bartonella quintana is known as the causative agent of trench fever. The disease caused major epidemics among soldiers in World Wars I and II. Humans are thought to be the unique natural reservoir for B. quintana, but this bacterium has also been isolated from captive-bred cynomolgus amd rhesus macaques in other countries. However, no epidemiological studies of B. quintana have been conducted in wild macaques.
The aim of the present study was to investigate the prevalence of B. quintana in wild Japanese macaques. In addition, we evaluated the potential pathogenicity of monkey-derived B. quintana for humans.
Bartonella quintana was isolated from six (13.3%) of 45 wild-caught Japanese macaques. The genetic characteristics of the isolates were found to be different from other known B. quintana strains derived from humans and captive-bred macaques. Moreover, it was suggested that the Japanese macaque strain has the potential to infect and invade into the human erythrocytes.

Research Products

• [Int'l Joint Research] University of California, Davis(米国)
• [Int'l Joint Research] University of Basel(Switzerland)
• [Journal Article] Japanese macaques (Macaca fuscata) as natural reservoir of Bartonella quintana (2015)
  • Author(s): Shingo Sato, Hidenori Kabeya, Aika Yoshino, Wataru Sekine, Kazuo Suzuki, Hidetoshi B. Tamate, Shouki Yamazaki, Bruno B. Chomel, Soichi Maruyama
  • Journal Title: Emerging Infectious Diseases Volume: 21 Issue: 12 Pages: 2168-2170
  • DOI: 10.3201/eid2112.150632
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] わが国の野生動物が保有するBartonella (2016)
  • Author(s): 丸山総一，佐藤真伍
  • Organizer: 第12 回日本獣医内科学アカデミー学術大会
  • Place of Presentation: 横浜市（パシフィコ横浜），神奈川県
  • Year and Date: 2016-02-19
  • Invited
• [Presentation] Japanese macaques (Macaca fuscata) as a new natural reservoir of Bartonella quintana, the causative agent of trench fever (2015)
  • Author(s): Shingo Sato, Hidenori Kabeya, Aika Yoshino, Wataru Sekine, Kazuo Suzuki, Hidetoshi B. Tamate, Shouki Yamazaki, Bruno B. Chomel, and Soichi Maruyama
  • Organizer: Third International Congress on Pathogens at the Human-Animal Interface (ICOPHAI)
  • Place of Presentation: Chiang Mai, Thailand
  • Year and Date: 2015-08-06
  • Int'l Joint Research
• [Presentation] わが国の野生ニホンザルのBartonella quintana保有状況と分離株の遺伝子解析 (2015)
  • Author(s): 佐藤真伍、壁谷英則、鈴木和男、東 英生、櫛引道彦、松岡史朗、山崎翔気、玉手英利、丸山総一
  • Organizer: 平成26年度 日本獣医師会獣医学術学会年次大会
  • Place of Presentation: 岡山コンベンションセンター(岡山県・岡山市)
  • Year and Date: 2015-02-14
• [Presentation] わが国の野生ニホンザルのBartonella quintana保有状況と分離株の遺伝子解析 (2014)
  • Author(s): 佐藤真伍、壁谷英則、吉野愛香、関根 渉、鈴木和男、東 英生、櫛引道彦、松岡史朗 山崎翔気、玉手英利、丸山総一
  • Organizer: 第157回日本獣医学会学術集会
  • Place of Presentation: 北海道大学（北海道・札幌市）
  • Year and Date: 2014-09-09