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Characterization of canine novel mesenchymal stem cells adhering to adipocytes in bone marrow for clinical application in regenerative therapy.

Research Project

Project/Area Number 26850192
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Veterinary medical science
Research InstitutionThe University of Tokyo

Principal Investigator

Fujita Naoki  東京大学, 大学院農学生命科学研究科(農学部), 特任助教 (10554488)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords間葉系幹細胞 / 犬 / 骨髄 / 再生医療 / 犬骨髄間葉系幹細胞 / 肝細胞成長因子 / 骨髄脂肪 / 幹細胞治療 / 骨髄間葉系幹細胞 / 脂肪細胞 / 肝細胞成長因子(HGF)
Outline of Final Research Achievements

We developed novel mesenchymal stem cells from canine bone marrow and named the cells as bone marrow per-adipocyte cells(BM-PACs). BM-PACs attached to adipocytes in bone marrow and have superior potentials as multipotent cells compared with conventional canine bone marrow mesenchymal stem cells (BMMSCs). Additionally, BM-PACs showed higher expression of hepatocyte growth factor (HGF), which is known to have great healing effects on injured tissues, than BMMSCs. After locally injected into a mouse skin injury model, BM-PACs migrated toward the injured area and showed HGF expression. In addition, intravenously injected BM-PACs also migrated toward injured area and showed HGF expression. Thus, our results suggest that stem cell therapy using BM-PACs offer a healing effect through the supply of HGF to the injured site.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (7 results)

All 2017 2016 2015 2014 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (5 results) (of which Int'l Joint Research: 1 results) Remarks (1 results)

  • [Journal Article] Isolation and Characterization of Multipotent Mesenchymal Stem Cells Adhering to Adipocytes in Canine Bone Marrow2017

    • Author(s)
      Lin Hsing-Yi, Fujita Naoki, Endo Kentaro, Morita Maresuke, Takeda Tae, Nakagawa Takayuki, and Nishimura Ryohei
    • Journal Title

      Stem Cells and Development

      Volume: 26 Issue: 6 Pages: 431-440

    • DOI

      10.1089/scd.2016.0200

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Transplantation of canine bone marrow peri-adipocyte cells for acute spinal cord injury in mice.2017

    • Author(s)
      林杏怡、藤田直己、遠藤健太郎、中川貴之、西村亮平
    • Organizer
      第16回日本再生医療学会
    • Place of Presentation
      仙台国際センター(宮城県仙台市)
    • Year and Date
      2017-03-06
    • Related Report
      2016 Annual Research Report
  • [Presentation] 間葉系幹細胞の効率的な軟骨分化条件の検討2017

    • Author(s)
      遠藤健太郎、藤田直己、林杏怡、中川貴之、西村亮平
    • Organizer
      第16回日本再生医療学会
    • Place of Presentation
      仙台国際センター(宮城県仙台市)
    • Year and Date
      2017-03-06
    • Related Report
      2016 Annual Research Report
  • [Presentation] TNF-α and IL-1β induce hepatocyte growth factor secretion by canine bone marrow peri-adipocyte cells2016

    • Author(s)
      林杏怡、藤田直己、遠藤健太郎、中川貴之、西村亮平
    • Organizer
      日本再生医療学会
    • Place of Presentation
      大阪
    • Year and Date
      2016-03-14
    • Related Report
      2015 Research-status Report
  • [Presentation] Ceilinf culture of adipose layer isolated from canine bone marrow can generate promising mesenchymal stem cells for regenerative medicine2015

    • Author(s)
      Lin Xing-Yi, Naoki Fujita, Kentaro Endo, Takayuki Nakagawa, Ryohei Nishimura
    • Organizer
      sian Meeting of. Animal Medical Specialtie
    • Place of Presentation
      Kuala Lumpur, Malaysia
    • Year and Date
      2015-10-31
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Ceiling culture of adipose lyaer isolated from canine bone marrow can generate promising mesenchymal stem cells for regenrative therapy2014

    • Author(s)
      Lin HY, Endo K, Fujita N, Nakagwa R, Nishimura R
    • Organizer
      Asian Meeting of Veterinary Surgery
    • Place of Presentation
      大阪
    • Year and Date
      2014-12-06 – 2014-12-07
    • Related Report
      2014 Research-status Report
  • [Remarks] 東京大学獣医外科学教室HP

    • URL

      http://www.vm.a.u-tokyo.ac.jp/geka/

    • Related Report
      2016 Annual Research Report 2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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