Development of a novel method to control translation by pseudorotaxane formation targeting on RNA
| Project/Area Number |
26860007
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemical pharmacy
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 核酸化学 / 人工核酸 / ロタキサン / 擬ロタキサン / DNA / RNA / 翻訳阻害 / 反応性DNA / クリックケミストリー |
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| Outline of Final Research Achievements |
It is expected that forming rotaxane-like architecture on target mRNA will lead to the regulation of gene expression. In this study, we tried to form pseudorotaxane architecture on the target RNA using a pair of pseudorotaxane-forming oligo DNAs (prfODNs) under physiological conditions. The pseudorotaxane formation reaction proceeded in 85% for 5 min, that is, we have developed the high-performance prfODN with high efficiency and yield.
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Report
(3 results)
Research Products
(12 results)
