Development of a novel method to control translation by pseudorotaxane formation targeting on RNA

• Project/Area Number: 26860007
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Chemical pharmacy
• Research Institution: Tohoku University
• Principal Investigator: Onizuka Kazumitsu 東北大学, 多元物質科学研究所, 助教 (00707961)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 核酸化学 / 人工核酸 / ロタキサン / 擬ロタキサン / DNA / RNA / 翻訳阻害 / 反応性DNA / クリックケミストリー

Outline of Final Research Achievements

It is expected that forming rotaxane-like architecture on target mRNA will lead to the regulation of gene expression. In this study, we tried to form pseudorotaxane architecture on the target RNA using a pair of pseudorotaxane-forming oligo DNAs (prfODNs) under physiological conditions. The pseudorotaxane formation reaction proceeded in 85% for 5 min, that is, we have developed the high-performance prfODN with high efficiency and yield.

Research Products

• [Journal Article] Automatic Pseudorotaxane Formation Targeting on Nucleic Acids Using a Pair of Reactive Oligodeox-ynucleotides (2014)
  • Author(s): Kazumitsu Onizuka*, Fumi Nagatsugi, Yoshihiro Ito, Hiroshi Abe
  • Journal Title: ournal of the American Chemical Society Volume: 136 Issue: 20 Pages: 7201-7204
  • DOI: 10.1021/ja5018283
  • Peer Reviewed
• [Presentation] 核酸を標的とした擬ロタキサン形成オリゴ核酸の構造最適化 (2016)
  • Author(s): 鬼塚 和光、宮下 卓也、雨宮 拓哉、永次 史
  • Organizer: 日本薬学会第136年会
  • Place of Presentation: パシフィコ横浜（横浜）
  • Year and Date: 2016-03-26
• [Presentation] 核酸標的擬ロタキサン形成オリゴDNAの構造最適化 (2016)
  • Author(s): 宮下 卓也、鬼塚 和光、永次 史
  • Organizer: 日本化学会第96春季年会
  • Place of Presentation: 同志社大学 京田辺キャンパス（京都）
  • Year and Date: 2016-03-24
• [Presentation] Development of pseudorotaxane formation methods targeting on nucleic acids (2015)
  • Author(s): Kazumitsu Onizuka, Yoshihiro Ito, Hiroshi Abe, Fumi Nagatsugi
  • Organizer: Pacifichem 2015
  • Place of Presentation: ハワイ（アメリカ合衆国）
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] Pseudorotaxane formation via slipping process targeting on nucleic acids (2015)
  • Author(s): Kazumitsu Onizuka, Takuya Miyashita , Fumi Nagatsugi
  • Organizer: The 42th International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: イーグレ姫路（姫路）
  • Year and Date: 2015-09-23
  • Int'l Joint Research
• [Presentation] 核酸を標的とした擬ロタキサン形成オリゴDNAの構造最適化 (2015)
  • Author(s): 宮下 卓也, 鬼塚 和光, 永次 史
  • Organizer: 第9回バイオ関連化学シンポジウム
  • Place of Presentation: 熊本大学（熊本）
  • Year and Date: 2015-09-10
• [Presentation] 化学反応性オリゴDNAによる擬ロタキサン形成反応 (2014)
  • Author(s): 鬼塚和光、伊藤嘉浩、阿部 洋、永次 史
  • Organizer: 第40回反応と合成の進歩シンポジウム
  • Place of Presentation: 東北大学
  • Year and Date: 2014-11-10 – 2014-11-11
• [Presentation] Automatic Pseudorotaxane Formation Targeting on Nucleic Acids (2014)
  • Author(s): Kazumitsu Onizuka, Yoshihiro Ito, Hiroshi Abe, Fumi Nagatsugi
  • Organizer: The 41st International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: Kitakyushu
  • Year and Date: 2014-11-05 – 2014-11-07
• [Presentation] Development of pseudorotaxane formation method targeting on nucleic acids (2014)
  • Author(s): Kazumitsu Onizuka, Yoshihiro Ito, Hiroshi Abe, Fumi Nagatsugi
  • Organizer: 3rd Switzerland-Japan Biomolecular Chemistry Symposium
  • Place of Presentation: Bern, Swizerland
  • Year and Date: 2014-10-02 – 2014-10-03
• [Presentation] 一対の化学反応性オリゴDNAによるRNA標的擬ロタキサン形成法 (2014)
  • Author(s): 鬼塚和光、伊藤嘉浩、阿部 洋、永次 史
  • Organizer: 第24回アンチセンスシンポジウム
  • Place of Presentation: 東京医科歯科大学
  • Year and Date: 2014-09-08 – 2014-09-09
• [Presentation] 反応性オリゴDNAを用いた擬ロタキサン形成法の開発 (2014)
  • Author(s): 鬼塚和光、伊藤嘉浩、阿部 洋、永次 史
  • Organizer: 日本ケミカルバイオロジー学会 第9回年会
  • Place of Presentation: 大阪大学
  • Year and Date: 2014-06-11 – 2014-06-13
• [Remarks] 永次研究室ホームページ
  • URL: http://www.tagen.tohoku.ac.jp/labo/nagatsugi/