|Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|Outline of Final Research Achievements
Conventional "GlycoPEGylation" is a synthetic method of glycoproteins attached PEG using glycosyltransferase. This strategy is very difficult to prepare a large amount of target compounds connected PEG owing to the use of glycosyltransferase. In order to resolve this problem, the development of "GlycoPEGylation" using a glycosylhydrolase represented by Endo-M was attempted.
A result of various studies, disaccharide oxazoline derivative which was consist of mannosyl-(β1-4)-N-acetyl glucosamine residues connected two PEG chains to 3’ and 6’ positions of the mannose residue was prepared and used as a glycosyl donor. Furthermore, subsequent enzymatic transglycosylation by using Endo-M was obtained a desired transglycosylated compound.