| Project/Area Number |
26860047
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biological pharmacy
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| Research Institution | Suzuka University of Medical Science |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | ビタミンK / UBIAD1 / 骨代謝 / 骨特異的遺伝子欠損マウス / タンパク質機能解析 / タンパク質構造解析 / 骨特異的ノックアウトマウス |
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| Outline of Final Research Achievements |
We have generated mice with bone-specific deficiency of noble vitamin K synthesis enzyme UBIAD1, using mice expressing Cre downstream of the osterix promoter that is specifically expressed in osteoblasts. The lengths and the weights of the generated mice exhibited significantly lower values as compared to those of wild type mice. Further, on performing a functional analysis of UBIAD1, four highly conserved regions were observed across the UBIAD1 variants. A point mutational analysis of UBIAD1 revealed changes in the amino acids involved in cholesterol synthesis and changes in the subcellular localization of UBIAD1. The obtained data has improved clarity on the structure-function relationship of UBIAD1 and the role of the UBIAD1 in bones.
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