Generation of mice with bone-specific deficiency of noble vitamin K synthesis enzyme UBIAD1 and elucidation of the physiological roles of UBIAD1.
| Project/Area Number |
26860047
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biological pharmacy
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| Research Institution | Suzuka University of Medical Science |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | ビタミンK / UBIAD1 / 骨代謝 / 骨特異的遺伝子欠損マウス / タンパク質機能解析 / タンパク質構造解析 / 骨特異的ノックアウトマウス |
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| Outline of Final Research Achievements |
We have generated mice with bone-specific deficiency of noble vitamin K synthesis enzyme UBIAD1, using mice expressing Cre downstream of the osterix promoter that is specifically expressed in osteoblasts. The lengths and the weights of the generated mice exhibited significantly lower values as compared to those of wild type mice. Further, on performing a functional analysis of UBIAD1, four highly conserved regions were observed across the UBIAD1 variants. A point mutational analysis of UBIAD1 revealed changes in the amino acids involved in cholesterol synthesis and changes in the subcellular localization of UBIAD1. The obtained data has improved clarity on the structure-function relationship of UBIAD1 and the role of the UBIAD1 in bones.
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Report
(3 results)
Research Products
(21 results)
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[Journal Article] Synthetic Small Molecules Derived from Natural Vitamin K Homologues that Induce Selective Neuronal Differentiation of Neuronal Progenitor Cells2015
Author(s)
Yoshitomo Suhara,Yoshihisa Hirota, Norika Hanada, Shun Nishina, Sachiko Eguchi, Rie Sakane, Kimie Nakagawa, Akimori Wada, Kazuhiko Takahashi, Hiroaki Tokiwa, and Toshio Okano
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Journal Title
J.Med.Chem.
Volume: 58 (17)
Issue: 17
Pages: 7088-7092
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Functional characterization of the vitamin K2 biosynthetic enzyme UBIAD12015
Author(s)
Hirota Y, Nakagawa K, Sawada N, Okuda N, Suhara Y, Uchino Y, Kimoto T, Funahashi N, Kamao M, Tsugawa N, Okano T.
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Journal Title
PLOS ONE.
Volume: 10
Issue: 4
Pages: e0125737-e0125737
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Vitamin K2 biosynthetic enzyme, UBIAD1 is essential for embryonic development of mice.2014
Author(s)
Nakagawa K, Sawada N, Hirota Y, Uchino Y, Suhara Y, Hasegawa T, Amizuka N, Okamoto T, Tsugawa N, Kamao M, Funahashi N, Okano T
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Journal Title
PLoS ONE.
Volume: 15;9(8)
Issue: 8
Pages: e104078-e104078
DOI
Related Report
Peer Reviewed / Open Access
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