Characterization of human fetal appendages-derived Muse cells as a new source of Muse cells for regenerative medicine

• Project/Area Number: 26860126
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General anatomy (including histology/embryology)
• Research Institution: Tohoku University
• Principal Investigator: Kushida Yoshihiro 東北大学, 医学(系)研究科(研究院), 助教 (10707003)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: Muse細胞 / 多能性幹細胞 / 間葉系幹細胞 / 胎児付属物

Outline of Final Research Achievements

Human umbilical cord tissue-derived Muse cells (UC-Muse cells) resided sparsely in Wharton’ jelly and expressed pluripotent markers such as Nanog and Oct3/4. These cells exhibited self-renewal and triploblastic differentiation under both spontaneous and induced differentiation condition. Furthermore, these cells didn’t develop into teratomas when transplanted into the testes of immunodeficient mice (SCID mice). These results suggest that UC-Muse cells exhibit the ability similar to that of adult human tissues and are useful as a new source of Muse cells for regenerative medicine.

Research Products

• [Journal Article] Induction of autoimmune gastritis by neonatal thymectomy requires autoantibodies and is prevented by anti-FcγR antibodies. (2016)
  • Author(s): Tsubasa SAITO, Satoru SUENAGA, Masato FUJII, Yoshihiro KUSHIDA, Yusuke KAWAUCHI, Kenji SUZUKI, Maki TOUMA, Masamichi HOSONO
  • Journal Title: Cellular Immunology Volume: 300 Pages: 1-8
  • DOI: 10.1016/j.cellimm.2015.10.004
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A distinct subpopulation of bone marrow mesenchymal stem cells, Muse cells, directly commit to the replacement of liver components. (2016)
  • Author(s): Katagiri H, Kushida Y, Nojima M, Kuroda Y, Wakao S, Ishida K, Endo F, Kume K, Takahara T, Nitta H, Tsuda H, Dezawa M, Nishizuka SS.
  • Journal Title: American Journal of Transplantation Volume: 16 Issue: 2 Pages: 468-483
  • DOI: 10.1111/ajt.13537
  • Peer Reviewed / Open Access
• [Journal Article] Transplantation of Unique Subpopulation of Fibroblasts, Muse Cells, Ameliorates Experimental (2016)
  • Author(s): Satow, T., Kawase, T., Kitamura, A. , Kajitani ,Y. , Yamaguchi, T. , Tanabe, N. , Otoi, R. , Komuro, T. , Kobayashi, A. , Nagata, H. , Mima, T.,
  • Journal Title: Stem Cells Volume: 34 Issue: 1 Pages: 160-173
  • DOI: 10.1002/stem.2206
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Experimental model of small subcortical infarcts in mice with long-lasting functional disabilities. (2015)
  • Author(s): Uchida H, Sakata H, Fujimura M, Niizuma K, Kushida Y, Dezawa M, Tominaga T
  • Journal Title: Brain Res. Volume: 1629 Pages: 318-328
  • DOI: 10.1016/j.brainres.2015.10.039
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Muse cells, a novel type of non-tumorigenic pluripotent stem cells, reside in human mesenchymal tissues (2014)
  • Author(s): Wakao S, Akashi H, Kushida Y, Dezawa M.
  • Journal Title: Pathol Int Volume: 64(1) Issue: 1 Pages: 1-9
  • DOI: 10.1111/pin.12129
  • Peer Reviewed
• [Journal Article] Population Doublings of Murine CD4+ memory T cells during continuous antigen stimulation in vivo. (2014)
  • Author(s): Kushida Y, Ishida J, Fujii M, Touma M, Hosono M.
  • Journal Title: Cell Immunol. Volume: 292 Issue: 1-2 Pages: 45-52
  • DOI: 10.1016/j.cellimm.2014.09.001
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A Dominant Trait Linked to Chromosome 1 in DBA/2 Mice for the Resistance to Autoimmune Gastritis Appears in Bone Marrow Cells (2014)
  • Author(s): Fujii M, Suzuki K, Suenaga S, Wakatsuki M, Kushida Y, Touma M, Hosono M.
  • Journal Title: Experimental Animals Volume: 63 Issue: 2 Pages: 155-167
  • DOI: 10.1538/expanim.63.155
  • NAID: 130003391616
  • ISSN: 0007-5124, 1341-1357, 1881-7122
  • Peer Reviewed / Open Access
• [Presentation] 臍帯組織中に存在する多能性幹細胞Muse細胞の自己複製能と多分化能の検証. (2016)
  • Author(s): 串田 良祐, 若尾 昌平, 明石 英雄, 西村 範行, 岩谷 壮太, 香田 翼, 森岡 一朗, 溝渕 雅巳, 飯島 一誠, 出澤 真理.
  • Organizer: 第121回日本解剖学会総会
  • Place of Presentation: 郡山、ビッグパレットふくしま
  • Year and Date: 2016-03-28
• [Presentation] 臍帯組織中に存在する多能性幹細胞Muse細胞の自己複製能と多分化能の検証. (2016)
  • Author(s): 串田 良祐, 若尾 昌平, 明石 英雄, 西村 範行, 岩谷 壮太, 香田 翼, 森岡 一朗, 溝渕 雅巳, 飯島 一誠, 出澤 真理.
  • Organizer: 第15回日本再生医療学会総会
  • Place of Presentation: 大阪、大阪国際会議場
  • Year and Date: 2016-03-17
• [Presentation] 臍帯組織中に存在する多能性幹細胞Muse細胞の多能性の解析. (2015)
  • Author(s): 串田 良祐, 若尾 昌平, 明石 英雄, 西村 範行, 岩谷 壮太, 香田 翼, 森岡 一朗, 溝渕 雅巳, 飯島 一誠, 出澤 真理.
  • Organizer: 第42回日本臓器保存生物医学会学術集会
  • Place of Presentation: 盛岡、いわて県民情報交流センター（アイーナ）
  • Year and Date: 2015-11-13
• [Presentation] 臍帯組織中に存在する多能性幹細胞Muse細胞の多能性の解析. (2015)
  • Author(s): 串田 良祐, 若尾 昌平, 明石 英雄, 西村 範行, 岩谷 壮太, 香田 翼, 森岡 一朗, 溝渕 雅巳, 飯島 一誠, 出澤 真理.
  • Organizer: 日本解剖学会第61回東北・北海道連合支部学術集会
  • Place of Presentation: 盛岡、盛岡市観光分化交流センタープラザおでって
  • Year and Date: 2015-08-29
• [Presentation] Analysis of pluripotency in Muse cells derived from human umbilical cord tissue. (2015)
  • Author(s): Kushida Y, Wakao S, Akashi H, Nishimura N, Iwatani S, Koda T, Morioka I, Mizobuchi M, Iijima K, Dezawa M.
  • Organizer: International Society for Stem Cell Research 2015 Annual Meeting
  • Place of Presentation: Sweden, Stockholm
  • Year and Date: 2015-06-24
  • Int'l Joint Research
• [Presentation] 臍帯組織由来の多能性幹細胞Muse細胞の探索と多能性の解析 (2015)
  • Author(s): 串田良祐、若尾昌平、明石英雄、西村範行、岩谷壮太、香田翼、森岡一朗、溝渕雅巳、飯島一誠、出澤真理
  • Organizer: 第14回日本再生医療学会
  • Place of Presentation: 神奈川県横浜市 パシフィコ横浜
  • Year and Date: 2015-03-19 – 2015-03-21
• [Presentation] 臍帯組織由来の多能性幹細胞Muse細胞の探索と多能性の解析 (2014)
  • Author(s): 串田良祐、若尾昌平、明石英雄、西村範行、岩谷壮太、香田翼、森岡一朗、溝渕雅巳、飯島一誠、出澤真理
  • Organizer: 日本解剖学会第60回東北・北海道連合支部学術集会
  • Place of Presentation: 福島県福島市 福島学院大学
  • Year and Date: 2014-09-06 – 2014-09-07
• [Remarks] 東北大学大学院医学系研究科細胞組織学分野
  • URL: http://www.stemcells.med.tohoku.ac.jp/index.html