Physiological function of D1 dopamine receptor-expressing astrocyte of substantia nigra

• Project/Area Number: 26860143
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General physiology
• Research Institution: Hirosaki University
• Principal Investigator: Nagatomo Katsuhiro 弘前大学, 医学研究科, 助教 (30542568)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: D1ドーパミン受容体 / アストロサイト / ドーパミン受容体 / 黒質網様部

Outline of Final Research Achievements

Substantia nigra pars reticulata (SNr) receives dopamine (DA) from dendrites (dendritic release) extending into this nucleus from dopaminergic neurons in the adjacent nucleus pars compacta, which is known to be selectively degenerated in Parkinson’s disease. Recently, we found acutely dissociated adult SNr astrocytes showed anti-D1 dopamine receptor (D1R) antibody immunoreactivity which might be as a recipient for dendritically released DA. Present study, the physiological function of D1R expressed in SNr astrocytes was examined by electrophysiological study in mouse brain slice and behavior analysis of SNr astrocyte-specific D1R-conditional knockout (D1R-CKO) mouse. The electrophysiological approach have yet given clues for the physiological function. A trouble on establishment of D1R-CKO mouse happened in the delivery process, then this approach could not be performed. The work for anti-D1R antibody immunoreactivity of acutely dissociated adult astrocyte was published on Feb., 2017.

Research Products

• [Journal Article] Dopamine D1 Receptor Immunoreactivity on Fine Processes of GFAP-Positive Astrocytes in the Substantia Nigra Pars Reticulata of Adult Mouse (2017)
  • Author(s): Katsuhiro Nagatomo, Sechiko Suga, Masato Saitoh, Masahito Kogawa, Kazuto Kobayashi, Yoshio Yamamoto, Katsuya Yamada
  • Journal Title: Frontiers in Neuroanatomy Volume: 11 Pages: 1-11
  • DOI: 10.3389/fnana.2017.00003
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Auto-oxidation products of epigallo-catechin gallate activate TRPA1 and TRPV1 in sensory neurons. (2015)
  • Author(s): Kurogi, M., Kawai, Y., Nagatomo, K., Tateyama, M., Kubo, Y. and Saitoh, O.
  • Journal Title: Chemical Senses Volume: 40(1) Issue: 1 Pages: 27-46
  • DOI: 10.1093/chemse/bju057
  • Peer Reviewed / Open Access
• [Journal Article] Singular localization of sodium channel β4 subunit in unmyelinated fibres and its role in the striatum. (2014)
  • Author(s): Miyazaki H, Oyama F, Inoue R, Aosaki T, Abe T, Kiyonari H, Kino Y, Kurosawa M, Shimizu J, Ogiwara I, Yamakawa K, Koshimizu Y, Fujiyama F, Kaneko T, Shimizu H, Nagatomo K, Yamada K, Shimogori T, Hattori N, Miura M, Nukina N.
  • Journal Title: Nat Commun. Volume: 5 Issue: 1 Pages: 5525-5525
  • DOI: 10.1038/ncomms6525
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] A hypothesis: dendro-dendritic communication mediated by astrocytic processes in substantia nigra (2015)
  • Author(s): Katsuya Yamada, Koji Shibasaki, Sechiko Suga, Yoshio Yamamoto, Makoto Tominaga, Katsuhiro Nagatomo
  • Organizer: 第38回日本神経科学大会
  • Place of Presentation: 神戸コンベンションセンター（兵庫県・神戸市）
  • Year and Date: 2015-07-30
• [Presentation] 大脳基底核におけるアストロサイトの領域特異的ドーパミン受容体発現 (2015)
  • Author(s): 山田 勝也、長友 克広
  • Organizer: 第92回日本生理学会大会
  • Place of Presentation: 神戸国際会議場・展示場(兵庫県・神戸市)
  • Year and Date: 2015-03-23