Evaluation of intracellular GPCR activation by using FRET imaging
Project/Area Number |
26860170
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
General pharmacology
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Research Institution | Asahikawa Medical College |
Principal Investigator |
Uwada Junsuke 旭川医科大学, 医学部, 助教 (70580314)
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Project Period (FY) |
2014-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | ムスカリン受容体 / FRET / GPCR / 薬理学 |
Outline of Final Research Achievements |
This research was aimed at elucidating the mechanism of activation of intracellular muscarinic M1 receptor using real-time visualization technique. Twelve FRET constructs of M1 receptor were designed and introduced to N1E-115 neuroblastoma cells. Effectiveness of each constructs is currently being examined using confocal microscopy. In addition, interaction of M1 receptor and associated G proteins were also examined in living cells. Interestingly, Gγ proteins was colocalized with M1 receptors at intracellular vesicles as well as at cell surface. On the other hand, C-terminal tail modified M1 receptor, which is exclusively localized intracellularly, showed poor colocalization with Gγ protein. Therefore, it was indicated that the coupling of intracellular M1 receptor and G proteins is dependent on the trafficking machinery of M1 receptors.
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Report
(3 results)
Research Products
(4 results)
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[Journal Article] Activation of muscarinic cholinoceptor ameliorates tumor necrosis factor-a-induced barrier dysfunction in intestinal epithelial cells.2015
Author(s)
Khan MR, Uwada J, Yazawa T, Islam MT, Krug SM, Fromm M, Karaki S, Suzuki Y, Kuwahara A, Yoshiki H, Sada K, Muramatsu I, Anisuzzaman AS and Taniguchi T.
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Journal Title
FEBS Lett.
Volume: 589
Issue: 23
Pages: 3640-3647
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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