| Project/Area Number |
26860170
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General pharmacology
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| Research Institution | Asahikawa Medical College |
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Principal Investigator |
Uwada Junsuke 旭川医科大学, 医学部, 助教 (70580314)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | ムスカリン受容体 / FRET / GPCR / 薬理学 |
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| Outline of Final Research Achievements |
This research was aimed at elucidating the mechanism of activation of intracellular muscarinic M1 receptor using real-time visualization technique. Twelve FRET constructs of M1 receptor were designed and introduced to N1E-115 neuroblastoma cells. Effectiveness of each constructs is currently being examined using confocal microscopy. In addition, interaction of M1 receptor and associated G proteins were also examined in living cells. Interestingly, Gγ proteins was colocalized with M1 receptors at intracellular vesicles as well as at cell surface. On the other hand, C-terminal tail modified M1 receptor, which is exclusively localized intracellularly, showed poor colocalization with Gγ protein. Therefore, it was indicated that the coupling of intracellular M1 receptor and G proteins is dependent on the trafficking machinery of M1 receptors.
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