Identification of the glycosylation and generation mechanisms of high-molecular weight type of lipocalin 2
| Project/Area Number |
26860173
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General pharmacology
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| Research Institution | Jichi Medical University |
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Principal Investigator |
FUJIWARA Yoko 自治医科大学, 医学部, ポスト・ドクター (50392494)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 腎障害 / バイオマーカー / 糖鎖 / 細胞極性 / 疾患動物モデル / 腎臓 / 薬剤性腎障害 / 薬物性腎障害 / 糖タンパク質 / 糖蛋白質 / 疾患モデル / 薬剤性腎症 / 炎症 |
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| Outline of Final Research Achievements |
Urinary glycoprotein lipocalin 2 (LCN2) has been noted as a sensitive biomarker for acute kidney injury. However, LCN2 could be detected in various disorders associated with the nephropathy since LCN2 is expressed in a variety of tissues. Here we try to identify high-molecular weight LCN2-producing tissues and clarify synthesis and secretion mechanisms of urine specific high-molecular weight LCN2. LCN2 was identified as complex-type glycan in urine, serum, and kidney in inflammatory model mice. On the other hand, hybrid-type glycosylated LCN2 was detected in liver. In addition, high-molecular weight LCN2 in urine was more glycosylated than that in serum and was appeared under intraperitoneal inflammation. These results indicate that LCN2 has a variety of glycosylation and the molecular weight of urinary LCN2 could differ among diseases.
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Report
(5 results)
Research Products
(5 results)
