Analysis of CD47-SIRPalpha system, an intercellular signaling system, in immune regulation

• Project/Area Number: 26860210
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pathological medical chemistry
• Research Institution: Kobe University
• Principal Investigator: Kotani Takenori 神戸大学, 医学(系)研究科(研究院), 助教 (40455960)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 細胞間シグナル伝達 / 樹状細胞 / チロシンリン酸化・脱リン酸化 / SIRPα / CD47 / 二次リンパ組織 / ストローマ細胞

Outline of Final Research Achievements

Signal regulatory protein α (SIRPα), an immunoglobulin superfamily protein that is expressed predominantly in dendritic cells (DCs) or macrophages, mediates cell-cell signaling by interacting with its ligand CD47, another immunoglobulin superfamily protein. In this study, I showed that mice lacking SIRPα specifically in DCs manifested a marked reduction of CD4-positive conventional DCs in the secondary lymphoid organs, as well as of Langerhans cells in the epidermis. Thus, these findings suggest that SIRPα on DCs is indispensable for homeostasis of DCs in the secondary lymphoid organs and skin.

Research Products

• [Journal Article] Dendritic cell SIRPα regulates homeostasis of dendritic cells in lymphoid organs (2015)
  • Author(s): Washio K, Kotani T, Saito Y, Respatika D, Murata Y, Kaneko Y, Okazawa H, Ohnishi H, Fukunaga A, Nishigori C, Matozaki T.
  • Journal Title: Genes Cells Volume: - Issue: 6 Pages: 00-00
  • DOI: 10.1111/gtc.12238
  • Peer Reviewed / Open Access
• [Journal Article] 【細胞シグナル操作法】分子からみたシグナル操作法 キナーゼ プロテインホスファターゼ(解説/特集) (2015)
  • Author(s): 小谷 武徳, 村田 陽二, 的崎 尚
  • Journal Title: 生体の科学 Volume: 66 Pages: 432-433
• [Presentation] Essential Role of SIRPα on Dendritic Cells in Homeostatic Regulation of Spleen Stromal Cells (2016)
  • Author(s): Datu Respatika, Yasuyuki Saito, Ken Washio, Satomi Komori, Takenori Kotani, Yoji Murata, Takashi Matozaki
  • Organizer: 第7回日本プロテインホスファターゼ研究会学術集会
  • Place of Presentation: 愛知県岡崎市(自然科学研究機構)
  • Year and Date: 2016-01-29
• [Presentation] Essential role of SIRPα on dendritic cells in homeostatic regulation of the secondary lymphoid organ (2015)
  • Author(s): Datu Respatika, Yasuyuki Saito, Ken Washio, Satomi Komori, Takenori Kotani, Yoji Murata, Takashi Matozaki
  • Organizer: 2015University of Washington and Kobe University joint symposium
  • Place of Presentation: University of Washington(Seattle, USA)
  • Year and Date: 2015-09-10
• [Presentation] Essential role of SIRPα on dendritic cells in organization and homeostasis of the spleen (2014)
  • Author(s): Ken Washio, Takenori Kotani, Datu Respatika, Yoji Murata, Yasuyuki Saito, Hideki Okazawa, Hiroshi Ohnishi, Atsushi Fukunaga, Chikako Nishigori, Takashi Matozaki
  • Organizer: 第43回日本免疫学会 学術集会
  • Place of Presentation: 京都府京都市（国立京都国際会館）
  • Year and Date: 2014-12-10
• [Presentation] 細胞間コミュニケーションシステムCD47-SIRPα系による血液・免疫系の制御/Regulation by CD47-SIRPα signaling of hematopoietic and immune systems (2014)
  • Author(s): 小谷武徳、鷲尾健、ダトゥレスパティカ、村田陽二、齊藤泰之、的崎尚
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: 神奈川県横浜市（パシフィコ横浜）
  • Year and Date: 2014-11-26
• [Presentation] 樹状細胞及び二次リンパ組織の恒常維持におけるSIRPαの役割 (2014)
  • Author(s): 鷲尾健、小谷武徳、レスパティカダトゥ、村田陽二、齊藤泰之、金子和光、岡澤秀樹、大西浩史、福永淳、錦織千佳子、的崎尚
  • Organizer: 第13回生体機能研究会
  • Place of Presentation: 岐阜県岐阜市（ホテルパーク）
  • Year and Date: 2014-07-19