Function and mechanism of exosome in neuroinflammation

Research Project

Project/Area Number	26860274
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Experimental pathology
Research Institution	Tokyo Metropolitan Geriatric Hospital and Institute of Gerontology
Principal Investigator	Kawakami Kyojiro 地方独立行政法人東京都健康長寿医療センター(東京都健康長寿医療センター研究所), 東京都健康長寿医療センター研究所, 研究員 (90589227)
Project Period (FY)	2014-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000) Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords	エクソソーム / ミクログリア / 神経炎症 / プロテオーム
Outline of Final Research Achievements	Neuroinflammation is profoundly involved in neurodegenerative diseases. We aim to elucidate the role of exosomes secreted from activated microglia in neuroinflammation. We found that protein composition in exosomes secreted from BV-2 microglia cells was altered by LPS treatment, among which histone molecules showed a remarkable increase. Treatment with histone molecules damaged PC12 neuronal cells, but exosomes derived from LPS-treated BV-2 cells showed no apparent cytotoxic effect. Further investigation using differentiated PC12 cells or primary cultured neurons will be required to elucidate the role of histones expressed on exosomes in neuroinflammation.