Research Project
Grant-in-Aid for Young Scientists (B)
We established the experimental method for expression and purification of tyrosine-phosphorylated CagA oncoprotein from Helicobacter pylori by employing bacterial expression system. Using the purified tyrosine-phosphorylated CagA protein, we elucidated that the interaction of CagA with Csk, one of the intracellular targets of CagA, is due to direct binding and that the CagA-bound Csk is aberrantly activated. Among multiple tyrosine-phosphorylation motifs in a single CagA molecule, we identified the key motif for the interaction of CagA with Csk. Furthermore, crystal structure analysis revealed that the interaction mode of CagA-Csk complex.
All 2016 2015 2014
All Journal Article (4 results) (of which Int'l Joint Research: 2 results, Peer Reviewed: 4 results, Open Access: 2 results, Acknowledgement Compliant: 3 results) Presentation (2 results) (of which Invited: 1 results) Book (1 results)
Biochemical and Biophysical Research Communications
Volume: 469 Issue: 4 Pages: 1133-1139
10.1016/j.bbrc.2015.12.117
Cryst. Growth Des.
Volume: 16 Issue: 3 Pages: 1565-1571
10.1021/acs.cgd.5b01692
Nature Microbiology
Volume: 1 Issue: 4 Pages: 16026-16026
10.1038/nmicrobiol.2016.26
Sci. Rep.
Volume: 5 Issue: 1 Pages: 15749-15749
10.1038/srep15749