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Analysis of bacteria-host neuron interactions to study the mechanism of whooping cough

Research Project

Project/Area Number 26860286
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Bacteriology (including mycology)
Research InstitutionOsaka University

Principal Investigator

Shinzawa Naoaki  大阪大学, 微生物病研究所, 助教 (10583015)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords百日咳 / 感覚神経 / カルシウムイメージング / 咳発作
Outline of Final Research Achievements

Whooping cough is a respiratory infectious disease caused by Bordetella pertussis. Infection results in a severe cough illness, however the mechanism is still uncovered. We hypothesized that direct stimulation of host tracheal sensory neuron by B. pertussis induce coughing during infection. To investigate this, we performed calcium imaging analysis to examine the interaction between bacterial lysates and rat sensory neurons. However, the specific neural stimulation by coughing-inducing bacterial lysates was not identified. In this work, we could not analyze the host neuron-bacteria interaction.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (7 results)

All 2016 2015

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results,  Acknowledgement Compliant: 2 results) Presentation (5 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] The bvg-repressed gene brtA, encoding biofilm-associated surface adhesin, is expressed during host infection by Bordetella bronchiseptica.2016

    • Author(s)
      Nishikawa S, Shinzawa N, Nakamura K, Ishigaki K, Abe H, Horiguchi Y.
    • Journal Title

      Microbiol Immunol.

      Volume: 60 Issue: 2 Pages: 93-105

    • DOI

      10.1111/1348-0421.12356

    • NAID

      40020747667

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Polymorphisms Influencing Expression of Dermonecrotic Toxin in Bordetella bronchiseptica.2015

    • Author(s)
      Okada K, Abe H, Ike F, Ogura Y, Hayashi T, Fukui-Miyazaki A, Nakamura K, Shinzawa N, Horiguchi Y.
    • Journal Title

      PLoS One

      Volume: 10 Issue: 2 Pages: e0116604-e0116604

    • DOI

      10.1371/journal.pone.0116604

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Development of the novel genomic integration system to study the host tropism of Bordetella spp.2016

    • Author(s)
      Ishigaki K, Nishikawa S, Shinzawa N, Horigichi Y
    • Organizer
      11th International Bordetella Symposium
    • Place of Presentation
      アルゼンチン・ブエノスアイレス
    • Year and Date
      2016-04-06
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] In vivo RNA-seq revealed infection-linked expression pattern of Bordetella pertussis within host2016

    • Author(s)
      新澤直明、鈴木孝一朗、元岡大祐、中村昇太、堀口安彦
    • Organizer
      第89回日本細菌学会総会
    • Place of Presentation
      大阪府
    • Year and Date
      2016-03-24
    • Related Report
      2015 Annual Research Report
  • [Presentation] 百日咳の病態発生機序解明に向けた感染動物モデルの確立2016

    • Author(s)
      中村佳司、神谷重樹、安倍裕順、新澤直明、堀口安彦
    • Organizer
      第89回日本細菌学会総会
    • Place of Presentation
      大阪府
    • Year and Date
      2016-03-23
    • Related Report
      2015 Annual Research Report
  • [Presentation] ボルデテラ属菌の宿主特異性決定機構解明に向けたゲノム相補システムの開発2016

    • Author(s)
      石垣佳祐、新澤直明、堀口安彦
    • Organizer
      第89回日本細菌学会総会
    • Place of Presentation
      大阪府
    • Year and Date
      2016-03-23
    • Related Report
      2015 Annual Research Report
  • [Presentation] Biofilm-associated Bordetella RTX surface adhesion (BrtA) is expressed during animal infection2015

    • Author(s)
      Nishikawa S, Shinzawa N, Ishigaki K, Abe H, Horigichi Y
    • Organizer
      The 14th Awaji International Forum on Infection and Immunity
    • Place of Presentation
      兵庫県
    • Year and Date
      2015-09-10
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research

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Published: 2014-04-04   Modified: 2017-05-10  

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