Study on the mechanism of action of novel biofilm inhibitor
| Project/Area Number |
26860294
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bacteriology (including mycology)
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| Research Institution | Jikei University School of Medicine |
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Principal Investigator |
OKUDA Kenichi 東京慈恵会医科大学, 医学部, 講師 (70624245)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | バイオフィルム / 黄色ブドウ球菌 / 低分子化合物 / 感染症 / スクリーニング / 細胞壁合成 / 化合物スクリーニング / ケミカルバイオロジー / 創薬 / 抗菌ペプチド |
|---|
| Outline of Final Research Achievements |
Biofilm formation by Staphylococcus aureus is involved in chronic medical device-associated infections. In this study, the mechanism of action of anti-biofilm compound-JK (JK1), a compound that inhibits biofilm formation by S. aureus, was analyzed. It was indicated that JK1 increases oxygen respiration activity of the cells and the activity is important for biofilm inhibition. Furthermore, JK1 increased susceptibility of S. aureus to aminoglycosides. It was also revealed that JK1 specifically binds to LytM, which is involved in cell wall turnover of S. aureus. Analysis using LytM mutants suggested that JK1 binds to the active center of LytM.
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Report
(5 results)
Research Products
(12 results)
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[Journal Article] Norgestimate inhibits staphylococcal biofilm formation and resensitizes methicillin-resistant Staphylococcus aureus to β-lactam antibiotics.2017
Author(s)
Yoshii Y, Okuda K, Yamada S, Nagakura M, Sugimoto S, Nagano T, Okabe T, Kojima H, Iwamoto T, Kuwano K, Mizunoe Y.
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Journal Title
NPJ Biofilms Microbiomes.
Volume: 3
Issue: 1
Pages: 18-18
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Identification of ABC-JK2, a small molecule inhibitor of Staphylococcal biofilm formation2016
Author(s)
Yutaka Yoshii, Ken-ichi Okuda, Satomi Yamada, Mari Nagakura, Shinya Sugimoto, Tetsuo Nagano, Takayoshi Okabe, Hirotatsu Kojima, Yoshimitsu Mizunoe
Organizer
Biofilms7
Place of Presentation
Porto, Portugal
Year and Date
2016-07-26
Related Report
Int'l Joint Research
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[Presentation] Identification of ABC-JK2, a small molecule inhibitor of Staphylococcal biofilm formation2016
Author(s)
Yutaka Yoshii, Ken-ichi Okuda, Satomi Yamada, Mari Nagakura, Shinya Sugimoto, Tetsuo Nagano, Takayoshi Okabe, Hirotatsu Kojima, Yoshimitsu Mizunoe
Organizer
ASM MICROBE 2016
Place of Presentation
Boston, USA
Year and Date
2016-06-16
Related Report
Int'l Joint Research
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