Development of monoclonal antibodies specific for H7 HA and their application to rapid detection of influenza A/H7N9 virus
Project/Area Number |
26860311
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Virology
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Research Institution | National Institute of Infectious Diseases |
Principal Investigator |
Takahashi Hitoshi 国立感染症研究所, インフルエンザウイルス研究センター, 主任研究官 (60555762)
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Project Period (FY) |
2014-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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Keywords | インフルエンザ / 診断 / H7N9 |
Outline of Final Research Achievements |
The avian influenza A/H7N9 virus caused first human infection in early 2013 in China. The H7N9 virus infection is sporadically occurring in humans and has the potential to cause serious future pandemic. To detect H7N9 virus sensitively and specifically by immunoassay, we produced monoclonal antibodies (mAbs) recognizing linear epitopes specific for H7 hemagglutinin (HA). When two peptides having conserved to linear epitopes of H7 HA immunized to mice, some mAb clones were obtained by the screening ELISA. These mAbs were reacted with H7 virus antigen specifically, not with other subtype virus antigens. The combination of these mAbs was specific for H7N9 virus and reacted most efficiently in sandwich ELISA.
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Report
(3 results)
Research Products
(14 results)
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[Journal Article] Novel Reassortant Avian Influenza A(H5N1) Virus in Human, Southern Vietnam, 20142016
Author(s)
I Takayama, NT Hieu, M Shirakura, M Nakauchi, S Fujisaki, H Takahashi, S Nagata, NT Long, T Odagiri, M Tashiro, T Kageyama
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Journal Title
Emerg Infect Dis
Volume: 22
Issue: 3
Pages: 557-559
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Development of a sensitive novel diagnostic kit for the highly pathogenic avian influenza A (H5N1) virus2014
Author(s)
Y Tsunetsugu-Yokota, K Nishimura, S Misawa, M Kobayashi-Ishihara, H Takahashi, I Takayama, K Ohnishi, S Itamura, H LK Nguyen, M TQ Le, G T Dang, L T Nguyen, M Tashiro and T Kageyama
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Journal Title
BMC Infect Dis
Volume: 14
Issue: 1
Pages: 362-362
DOI
Related Report
Peer Reviewed / Open Access
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