| Project/Area Number |
26860364
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory medicine
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| Research Institution | Shinshu University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 蛍光磁性ビーズ / 疾患マーカー / 免疫測定 / 競合測定 / 同時多項目測定 |
|---|
| Outline of Final Research Achievements |
For improvement of rapidity, sensitivity and specificity of disease diagnosis, we aimed development of a concurrent multiple disease marker immunoassay using fluorescent magnetic beads. First, we developed several fluorescent magnetic beads encapsulating different fluorescent pigments respectively. Next, we constructed a competitive immunoassay using a combination of antibody immobilized fluorescent magnetic beads and an antigen coated plate. Furthermore, using two different colored fluorescent magnetic beads, we concurrently measured two disease markers. As the result of a fluorescent crosstalk correction, the net concentration of each disease maker was correctly measured. Using a magnetic collection of fluorescent magnetic beads, the concurrent multiple immunoassay detected the disease markers within 10 min.
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