| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
There are 36 kinds of amyloid precursor proteins that make amyloid fibrils. Amyloidosis is classified as each disease according to type of amyloid precursor protein, and the treatment is different. For this reason, it is extremely important to accurately identify the amyloid precursor protein for typing of amyloidosis. In this study, we developed a new extraction method for amyloid and establish a system that can accurately identify amyloid precursor protein. In addition, we tried to pick up candidates as prognostic markers using mass spectrometry for AL amyloidosis.
It revealed that the analytical method using proteinase K increased the detection rate of amyloid precursor protein. In AL amyloidosis, many type of peptides derived from L chain could be detected by using mass spectrometry. In addition, candidate peptides may become a prognostic marker for AL amyloidosis.
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