Associtation circulating miRNAs and lifestyle : aiming at application to clinical examination

• Project/Area Number: 26860375
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Laboratory medicine
• Research Institution: Fujita Health University
• Principal Investigator: Yamada Hiroya 藤田保健衛生大学, 医学部, 講師 (80610352)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: microRNA / Circulating miRNA / Serum miRNA / 遺伝子検査 / 生活習慣 / 変動要因 / バイオマーカー / 臨床検査 / circulating miRNA / 生理的変動要因

Outline of Final Research Achievements

Circulating micro (mi)RNA profiles are influenced by various medical conditions, and miRNAs have been examined as potential biomarkers for cancer, cardiovascular disease, and diabetes. However, few studies have investigated whether circulating miRNAs and life style factors, such as cigarette smoking and drinking, are correlated. Our aim was to determine the association between smoking status and expression of various miRNAs in a Japanese population sample. We found that life style factors is associated with elevated expression of various serum miRNAs.
Our results suggest that it is necessary to consider the confounding effect caused by lifestyle when evaluating expression of serum miRNAs for diagnosing pathological conditions.

Research Products

• [Journal Article] Stability of serum high-density lipoprotein-microRNAs for preanalytical conditions. (2017)
  • Author(s): Ishikawa H, Yamada H, Taromaru N, Kondo K, Nagura A, Yamazaki M, Ando Y, Munetsuna E, Suzuki K, Ohashi K, Teradaira R.
  • Journal Title: Ann Clin Biochem Volume: 54 Issue: 1 Pages: 134-142
  • DOI: 10.1177/0004563216647086
  • Peer Reviewed / Open Access
• [Journal Article] Circulating microRNAs in Graves' Disease in Relation to Clinical Activity. (2016)
  • Author(s): Hiratsuka I, Yamada H, Munetsuna E, Hashimoto S, Itoh M.
  • Journal Title: Thyroid Volume: 26 Pages: 1431-1440
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Association of cigarette smoking with serum microRNA expression (2016)
  • Author(s): Koji Suzuki, Hiroya Yamada, Ayuri Nagura, Koji Ohashi, Hiroaki Ishikawa, Mirai Yamazaki,
  • Journal Title: Fujita Medical Journal Volume: 1 Pages: 1-5
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Longitudinal study of circulating miR-122 in a rat model of non-alcoholic fatty liver disease. (2015)
  • Author(s): Yamada H, Ohashi K, Suzuki K, Munetsuna E, Ando Y, Yamazaki M, Ishikawa H, Ichino N, Teradaira R, Hashimoto S.
  • Journal Title: Clin Chim Acta. Volume: 446 Pages: 267-271
  • DOI: 10.1016/j.cca.2013.05.021
  • Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Simultaneous measurement of serum chemokines in autoimmune thyroid diseases: possible role of IP-10 in the inflammatory response (2015)
  • Author(s): Hiratsuka I, Itoh M, Yamada H, Yamamoto K, Tomatsu E, Makino M, Hashimoto S, Suzuki A.
  • Journal Title: Endocrine Journal Volume: 62 Issue: 12 Pages: 1059-1066
  • DOI: 10.1507/endocrj.EJ15-0448
  • NAID: 130005115211
  • ISSN: 0918-8959, 1348-4540
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 新規バイオマーカーとしての分泌型microRNAと臨床検査への応用 (2015)
  • Author(s): 山田宏哉
  • Organizer: 第55回日本臨床化学会
  • Place of Presentation: 大阪大学
  • Year and Date: 2015-10-30
  • Invited
• [Presentation] 住民健診受診者における血清miRNA値と皮下脂肪・内臓脂肪との関連 (2015)
  • Author(s): 山崎 未来, 山田 宏哉, 鈴木 康司, 市野 直浩, 石川 浩章, 刑部 恵介, 杉本 恵子, 宗綱 栄二, 近藤 奏子, 水野 元基, 村瀬 悠理, 井上 孝, 寺平 良治, 大橋 鉱二
  • Organizer: 第55回日本臨床化学会
  • Place of Presentation: 大阪大学
  • Year and Date: 2015-10-30
• [Presentation] 室温における全血放置が血清miRNA値に及ぼす影響についての検討 (2015)
  • Author(s): 近藤 奏子, 山崎 未来, 村瀬 悠理, 水野 元貴, 山田 宏哉, 宗綱 栄二, 石川 浩章, 鈴木 康司, 寺平 良治, 大橋 鉱二
  • Organizer: 第55回日本臨床化学会
  • Place of Presentation: 大阪大学
  • Year and Date: 2015-10-30
• [Presentation] 新規バイオ マーカーとしての血中 microRNA と臨床検査への応用 (2015)
  • Author(s): 山田宏哉
  • Organizer: 日本臨床化学会 近畿支部総会
  • Place of Presentation: 大阪
  • Year and Date: 2015-02-14
  • Invited