| Project/Area Number |
26860578
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cardiovascular medicine
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
KAMI DAISUKE 京都府立医科大学, 医学(系)研究科(研究院), 講師 (80415588)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
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| Keywords | Long non-coding RNA / 心筋細胞 / iPS細胞 / 心筋分化誘導 / Heart development / iPSC / Long Non-coding RNA / 心筋分化 |
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| Outline of Final Research Achievements |
Long non-coding RNA (lncRNA) performs the regulation in the cell, and it is also involved in the mouse heart development. On the other hand, the involvement of lncRNA in human heart development remain unclear. Therefore, we induced differentiation into cardiomyocytes using human iPS cells and RNA-seq analysis was performed in a time dependent. As a result, lncRNA EVX1 AS was identified. Over expression of EVX1AS in iPS cells increased the expression level of cardiomyocyte-related genes such as GATA4, MEF2C, and TBX5.However, its functions are still unknown and it is necessary to continue the analysis in the future.
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