Development of new rapid PCR method for viral respiratory tract infections

• Project/Area Number: 26860608
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Respiratory organ internal medicine
• Research Institution: Tottori University
• Principal Investigator: TAKATA MIYAKO 鳥取大学, 医学部, プロジェクト研究員 (50523643)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: PCR / インフルエンザ / 超高速PCR

Outline of Final Research Achievements

Immunological testing method for infectious disease, such as influenza virus is simple and quickly.However, this method have problem with limit of detection.This study revealed that development of high sensitivity PCR method.In particular, there is possibility that flu test showed negative and patient wasn't prescribed appropriate medicine.Establishment of high sensitivity detecting method provide with prevention in the sympton aggravation for compromised patient and the infection from spreading.This study, flu test showed negative but PCR method showed positive. In short, PCR method is effective for early stage.