Analysis of novel transcriptional mechanisms and identification of non-coding RNAs contributing renal fibrosis.
| Project/Area Number |
26860627
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Kidney internal medicine
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| Research Institution | The University of Tokyo |
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Principal Investigator |
MIMURA IMARI 東京大学, 医学部附属病院, 助教 (00727084)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 腎線維化 / 非翻訳RNA / 腎尿細管間質 / 線維化 / ヒストン / non-coding RNA / 慢性腎不全 / 低酸素 |
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| Outline of Final Research Achievements |
The main researcher of this project administered Dznep, which is one of inhibitors of histone repressive mark H3K27me3, to chronic kidney disease model mice in order to identify novel epigenetic mechanisms and factors which contribute to the reduction of tubulointerstitial fibrosis. I used laser captured microdissection to cut out only tubular cells from in vivo mice cortexes. I performed genome-wide analysis of RNA using high throughput sequencers. As a result, I identified changes of genes or lincRNAs expressions which are associated with renal fibrosis. These results suggested that there is a possibility that epigenetic factors driven by Dznep lead to amelioration of tubulointerstitial fibrosis.
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Report
(3 results)
Research Products
(25 results)
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[Journal Article] Quantitating intracellular oxygen tension in vivo by phosphorescence lifetime measurement2015
Author(s)
Hirakawa Y, Yoshihara T, Kamiya M, Mimura I, Fujikura D, Masuda T, Kikuchi R, Takahashi I, Urano Y, Tobita S, Nangaku M.
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Journal Title
Sci Rep
Volume: 5
Issue: 1
Pages: 17838-17838
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Cross-enhancement of ANGPTL4 transcription by HIF1 alpha and PPAR beta/delta is the result of the conformational proximity of two response elements.2014
Author(s)
Inoue T, Kohro T, Tanaka T, Kanki Y, Li G, Poh HM, Mimura I, Kobayashi M, Taguchi A, Maejima T, Suehiro JI, Sugiyama A, Kaneki K, Aruga H, Dong S, Stevens JF, Yamamoto S, Tsutsumi S, Fujita T, Ruan X, Aburatani H, Nangaku M, Ruan Y, Kodama T, Wada Y.
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Journal Title
Genome Biology
Volume: 15
Issue: 4
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Direct Evidence for Pitavastatin Induced Chromatin Structure Change in the KLF4 Gene in Endothelial Cells.2014
Author(s)
Maejima T, Inoue T, Kanki Y, Kohro T, Li G, Ohta Y, Kimura H, Kobayashi M, Taguchi A, Tsutsumi S, Iwanari H, Yamamoto S, Aruga H, Dong S, Stevens JF, Poh HM, Yamamoto K, Kawamura T, Mimura I,
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Journal Title
PLoS One.
Volume: 9
Issue: 5
Pages: e96005-e96005
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Endothelin-converting enzyme is a plausible target gene for hypoxia-inducible factor.2014
Author(s)
Khamaisi M, Toukan H, Axelrod JH, Rosenberger C, Skarzinski G, Shina A, Meidan R, Koesters R, Rosen S, Walkinshaw G, Mimura I, Nangaku M, Heyman SN.
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Journal Title
Kidney International
Volume: 87
Issue: 4
Pages: 761-770
DOI
Related Report
Peer Reviewed
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[Presentation] Quantitating intracellular oxygen tension in kidney by phosphorescence lifetime measurement.2015
Author(s)
Yosuke, Hirakawa, Imari, Mimura, Toshitada, Yoshihara, Mako, Kamiya, Yasuteru, Urano, Seiji, Tobita, Masaomi, Nangaku
Organizer
American Society of Nephrology
Place of Presentation
SanDiego（アメリカ合衆国）
Year and Date
2015-11-03
Related Report
Int'l Joint Research
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[Presentation] Estimation of intracellular oxygen concentration in diabetic kidney by phosphorescence lifetime measurement2015
Author(s)
Yosuke, Hirakawa, Imari, Mimura, Toshitada, Yoshihara, Mako, Kamiya, Yasuteru, Urano, Seiji, Tobita, Masaomi, Nangaku
Organizer
Keystone Symposia 2015
Place of Presentation
ウェスティン都ホテル京都（京都府京都市）
Year and Date
2015-10-25
Related Report
Int'l Joint Research
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