Functional analysis of transcription factor Rfx6 in GIP-producing K-cell
Project/Area Number |
26860695
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Metabolomics
|
Research Institution | Kyoto University |
Principal Investigator |
YAMANE Shunsuke 京都大学, 医学(系)研究科(研究院), 助教 (90582156)
|
Project Period (FY) |
2014-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | インクレチン / 肥満 / 肥満症 / GIP |
Outline of Final Research Achievements |
To investigate the hypothesis that Rfx6 and Rfx3 cooperate with each other and are involved in the regulation of GIP expression in K-cell, we evaluated the effect of knockdown and overexpression of Rfx6 and Rfx3 on GIP gene expression using STC-1 cell line. GIP mRNA expression levels were significantly decreased in Rfx6- knockdown STC-1 cells as we have previously reported. However, Rfx3 knockdown had no effect on GIP gene expression. In addition, simultaneous knockdown of Rfx6 and Rfx3 in STC-1 cells resulted in no change in GIP gene expression compared to single knockdown of Rfx6. Increased GIP expression levels were observed in Rfx6-overexpressiing STC-1 cells and GIP expression levels in Rfx6/Rfx3- overexpressing cells were similar to those in Rfx6-overexpressiing STC-1 cells.
|
Report
(3 results)
Research Products
(13 results)
-
-
[Journal Article] Enteral supplement enriched with glutamine, fiber, and oligosaccharide modulates incretin and glucagon-like peptide-2 secretion.2015
Author(s)
Joo E., Muraoka A., Hamasaki A., Harada N., Yamane S., Kondo Y., Suzuki K., Nasteska D., Shibue K., Harada T., Iwasaki K., Tsuji H., Shide K., Inagaki N.
-
Journal Title
J Diabetes Investig.
Volume: 6
Issue: 3
Pages: 302-308
DOI
Related Report
Peer Reviewed / Open Access
-
[Journal Article] Fatty acid binding protein 5 (FABP5) regulates diet-induced obesity (DIO) via GIP secretion from enteroendocrine K-cells in response to fat ingestion.2015
Author(s)
Shibue, K., Yamane, S., Harada, N., Hamasaki, A., Suzuki, K., Joo, E., Iwasaki, K., Nasteska, D., Harada, T., Hayashi, Y., Adachi, Y., Owada, Y., Takayanagi, R. and Inagaki, N.
-
Journal Title
Am J Physiol Endocrinol Metab
Volume: 308
Issue: 7
Pages: 583-591
DOI
Related Report
Peer Reviewed / Open Access
-
[Journal Article] Free fatty acid receptor GPR120 is highly expressed in enteroendocrine K-cells of upper small intestine and has a critical role in GIP secretion after fat ingestion.2015
Author(s)
Iwasaki K, Harada N, Sasaki K, Yamane S, Iida K, Suzuki K, Hamasaki A, Joo E, Nasteska D, Shibue K, Harada T, Hashimoto T, Asakawa Y, Hirasawa A, Inagaki N.
-
Journal Title
Endocrinology
Volume: 156
Issue: 3
Pages: 837-837
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
-
[Presentation] Inhibition of GIP receptor signaling in adipose tissue reduces insulin resistance and improves hepatic steatosis in high fat diet-induced obesity2015
Author(s)
Erina Joo,Norio Harada, Shunsuke Yamane, Toru Fukushima, Daisuke Taura, Kanako Iwasaki, Akiko Sankoda, Yuki Murata, Kimitaka Shibue, Kazuyo Suzuki, Takanari Harada, Satoko Kuwahara, Yuta Fujiwara, Nobuya Inagaki
Organizer
Incretin 2015
Place of Presentation
Vancouver, Canada
Year and Date
2015-07-31
Related Report
Int'l Joint Research
-
[Presentation] Fatty acid-binding protein 5 regulates diet-induced obesity via GIP secretion from enteroendocrine K-cells in response to fat ingestion2015
Author(s)
Kimitaka Shibue,Shunsuke Yamane,Norio Harada, Akihiro Hamasaki, Kazuyo Suzuki, Erina Joo, Kanako Iwasaki, Daniela Nasteska, Takanari Harada, Yoshitaka Hayashi, Yasuhiro Adachi, Yuji Owada, Ryoichi Takayanagi, Nobuya Inagaki
Organizer
Incretin 2015
Place of Presentation
Vancouver, Canada
Year and Date
2015-07-31
Related Report
Int'l Joint Research
-
[Presentation] Free fatty acid receptor GPR120 is highly expressed in enteroendocrine K-cells of upper small intestine and has a critical role in GIP secretion after fat ingestion2015
Author(s)
Takanari Harada, Kanako Iwasaki,Norio Harada, Kazuki Sasaki, Shunsuke Yamane, Keiko Iida, Kazuyo Suzuki, Akihiro Hamasaki, Daniela Nasteska, Kimitaka Shibue, Erina Joo,Toshihiro Hashimoto, Yoshinori, Asakawa, Akira Hirasawa, Nobuya Inagaki
Organizer
Incretin 2015
Place of Presentation
Vancouver, Canada
Year and Date
2015-07-31
Related Report
Int'l Joint Research
-
[Presentation] Fatty acid-binding protein 5 regulates diet-induced obesity via GIP secretion from enteroendocrine K cells in response to fat ingestion2015
Author(s)
Kimitaka Shibue,Shunsuke Yamane,Norio Harada,Akihiro Hamasaki,Kazuyo Suzuki, Erina Joo, Kanako Iwasaki, Daniela Nasteska, Takanari Harada, Yoshitaka Hayashi, Yasuhiro Adachi, Yuji Owada, Ryoichi Takayanagi, Nobuya Inagaki
Organizer
The American Diabetes Association’s 75th Scientific Sessions
Place of Presentation
Boston, MA
Year and Date
2015-06-08
Related Report
Int'l Joint Research
-
-
-
-
-
[Presentation] FABP5 is an essential modulator of fatty acid-induced GIP secretion in enteroendocrine K cells2014
Author(s)
Kimitaka Shibue, Shunsuke Yamane, Norio Harada, Akihiro Hamasaki, Kazuyo Suzuki, Erina Joo, Kanako Iwasaki, Daniela Nasteska Takanari Harada, Yasuhiro Adachi, Yuji Owada, Ryoichi Takayanagi, Nobuya Inagaki
Organizer
50th EASD Annual Meeting
Place of Presentation
Wien, Austria
Year and Date
2014-09-16 – 2014-09-19
Related Report