The novel mechanism of aldosterone production in aldosterone-producing adenoma
| Project/Area Number |
26860707
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Endocrinology
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| Research Institution | Hiroshima University |
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Principal Investigator |
OKI KENJI 広島大学, 病院(医), 病院助教 (30638995)
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| Research Collaborator |
Gomez-Sanchez Celso E. University of Mississippi Medical Center
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 原発性アルドステロン症 / アルドステロン / G蛋白共役型受容体 / アルドステロン合成酵素 / 細胞内カルシウム伝達 / G蛋白共役受容体 |
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| Outline of Final Research Achievements |
Microarray analysis targeting GPCR-associated genes was conducted using APA without known mutations (APA-WT) samples and APA with the KCNJ5 mutation (APA-KCNJ5). Gonadotropin-releasing hormone receptor (GNRHR) was the highest expression in APA-WT by microarray analysis. The quantitative polymerase chain reaction (qPCR) assay results revealed higher GNRHR expression levels in APA-WT samples those in APA-KCNJ5 samples. Patients with APA-WT (n=9), which showed higher GNRHR and LHCGR levels, had significantly higher GnRH-stimulated aldosterone response than those with APA-KCNJ5 (n=13) (P<0.05). HAC15 cells with KCNJ5 gene carrying T158A mutation exhibited a significantly lower GNRHR expression than that in control cells. We clarified increased expression of GNRHR and LHCGR in APA-WT, and the molecular analysis including the receptor expression associated with clinical findings of GnRH stimulation.
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Report
(4 results)
Research Products
(26 results)
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[Journal Article] Immunohistochemistry of aldosterone synthase leads the way to the pathogenesis of primary aldosteronism2017
Author(s)
Nishimoto, K., Koga, M., Seki, T., Oki, K., Gomez-Sanchez, E.P., Gomez-Sanchez, C.E., Naruse, M., Sakaguchi, T., Morita, S., Kosaka, T., Oya, M., Ogishima, T., Yasuda, M., Suematsu, M., Kabe, T., Omura, M., Nishikawa, T., Mukai, K.
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Journal Title
Mol. Cell. Endocrinol.
Volume: 441
Pages: 124-133
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Presentation] Ca2+ Binding Protein, Calneuron 1, Increased Ca2+ in Endoplasmic Reticulum and Aldosterone Production in Aldosterone-producing Adenoma2017
Author(s)
Kazuhiro Kobuke, Kenji Oki, Celso E. Gomez-Sanchez, Elise P. Gomez-Sanchez, Kiyotaka Itcho, Yoko Yoshii, Haruya Ohno, Masayasu Yoneda
Organizer
42nd Meeting of the International Aldosterone Conference
Place of Presentation
Orlando, FL, USA
Year and Date
2017-03-30
Related Report
Int'l Joint Research
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[Presentation] The Relationship Between Tumor Genotype and Clinical or Pathological Characteristics in Patients with Aldosterone Producing Adenoma2015
Author(s)
Kiyotaka Itcho, Kazuhiro Kobuke, Kenji Oki, Rui Kishimoto, Shusaku Maeda, Haruya Ohno, Masayasu Yoneda, Nobuoki Kohno
Organizer
The Endocrine society's 96th Annual Meeting and Expo
Place of Presentation
McCormick Place, Chicago, IL, USA
Year and Date
2015-06-21 – 2015-06-24
Related Report
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[Presentation] The clinical and pathological characteristics of aldosterone producing adenoma with ATP1A1 mutation2015
Author(s)
Kazuhiro Kobuke, Kiyotaka Itcho, Kenji Oki, Rui Kishimoto, Shusaku Maeda, Haruya Ohno, Masayasu Yoneda, Nobuoki Kohno
Organizer
40th Meeting of the International Aldosterone Meeting
Place of Presentation
The Westin Chicago River North, Chicago, IL, USA
Year and Date
2015-06-19 – 2015-06-20
Related Report
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