Research Project
Grant-in-Aid for Young Scientists (B)
Microarray analysis targeting GPCR-associated genes was conducted using APA without known mutations (APA-WT) samples and APA with the KCNJ5 mutation (APA-KCNJ5). Gonadotropin-releasing hormone receptor (GNRHR) was the highest expression in APA-WT by microarray analysis. The quantitative polymerase chain reaction (qPCR) assay results revealed higher GNRHR expression levels in APA-WT samples those in APA-KCNJ5 samples. Patients with APA-WT (n=9), which showed higher GNRHR and LHCGR levels, had significantly higher GnRH-stimulated aldosterone response than those with APA-KCNJ5 (n=13) (P<0.05). HAC15 cells with KCNJ5 gene carrying T158A mutation exhibited a significantly lower GNRHR expression than that in control cells. We clarified increased expression of GNRHR and LHCGR in APA-WT, and the molecular analysis including the receptor expression associated with clinical findings of GnRH stimulation.
All 2017 2016 2015 Other
All Int'l Joint Research (2 results) Journal Article (5 results) (of which Int'l Joint Research: 5 results, Peer Reviewed: 5 results, Open Access: 5 results, Acknowledgement Compliant: 4 results) Presentation (19 results) (of which Int'l Joint Research: 4 results)
Mol. Cell. Endocrinol.
Volume: 441 Pages: 124-133
10.1016/j.mce.2016.10.014
Medicine (Baltimore)
Volume: 95 Issue: 20 Pages: e3659-e3659
10.1097/md.0000000000003659
Mol Cell Endocrinol
Volume: 434 Pages: 93-98
10.1016/j.mce.2016.06.022
Endocrine Journal
Volume: 63 Issue: 2 Pages: 119-126
10.1507/endocrj.EJ15-0360
130005127189
Hypertension
Volume: 68 Issue: 6 Pages: 1432-1437
10.1161/hypertensionaha.116.08313
