The mechanism of erythroid lineage caused by Myh9 R702C mutation

• Project/Area Number: 26860725
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Hematology
• Research Institution: Nagoya University
• Principal Investigator: Suzuki Nobuaki 名古屋大学, 医学部附属病院, 助教 (70637686)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: MYH9 / 赤血球 / 赤血球文化 / MYH9異常症 / マウスモデル / 造血機能

Outline of Final Research Achievements

We generated hematopoietic stem cell(HSC)-specific Myh9 R702C mice, and estimated red blood cells (RBCs) and cells of the erythroid lineage. HSC-specific Myh9 R702C mice displayed mild anemia and high erythropoietin level compared with wild type mice. However, we could not find no difference in the character of RBC and differentiation of erythroid lineage compared with wild type mice in this research.

Research Products

• [Journal Article] A case of MYH9 disorders caused by a novel mutation (p.K74E). (2016)
  • Author(s): Kanematsu T, Suzuki N, Yoshida T, Kishimoto M, Aoki T, Ogawa M, Kagami Y, Kiyoi H, Matsushita T, Kunishima S.
  • Journal Title: Ann Hematol. Volume: 95(1) Issue: 1 Pages: 161-3
  • DOI: 10.1007/s00277-015-2506-9
  • Peer Reviewed / Open Access
• [Journal Article] The features of clearance in recombinant factor IX (BeneFIX). (2015)
  • Author(s): Suzuki N, Takedani H, Hirakawa A, Ushijima Y, Matsushita T.
  • Journal Title: Haeophilia Volume: in press Issue: 5 Pages: 702-707
  • DOI: 10.1111/hae.12672
  • Peer Reviewed
• [Journal Article] Endoscopic treatment of esophageal varices in hemophiliac patients with liver cirrhosis (2015)
  • Author(s): Ishizu Y, Ishigami M, Suzuki N, Kuzuya T, Honda T, Hayashi K, Katano Y, Hirooka Y, Ishikawa T, Matsushita T, Goto H.
  • Journal Title: Gastrointest Endosc Volume: 81 Issue: 4 Pages: 1059-1060
  • DOI: 10.1016/j.gie.2014.11.043
  • Peer Reviewed / Open Access
• [Journal Article] Rhof promotes murine marginal zone B cell development (2014)
  • Author(s): Kishimoto M, Matsuda T, Yanase S, Katsumi A, Suzuki N, Ikejiri M, Takagi A, Ikawa M, Kojima T, Kunishima S, Kiyoi H, Naoe T, Matsushita T, Maruyama M.
  • Journal Title: Nagoya J Med Sci Volume: 76 Pages: 293-305
  • NAID: 120005468127
  • Peer Reviewed / Open Access
• [Journal Article] Hepatectomy for hepatocellular carcinoma in patients with hemophilia. (2014)
  • Author(s): Inokawa Y, Sugimoto H, Kanda M, Yamada S, Fujii T, Nomoto S, Takeda S, Suzuki N, Matsushita T, Kodera Y.
  • Journal Title: J Hepatobiliary Pancreat Sci Volume: 51 Issue: 12 Pages: 824-828
  • DOI: 10.1007/s13197-012-0910-x
  • Peer Reviewed
• [Presentation] 末期腎不全の経過中、家族歴を有する巨大血小板減少症に気付かれ、新規p.K74E変異によるMYH9異常症と診断した1例 (2015)
  • Author(s): 兼松毅, 鈴木 伸明, 小川 実加, 岸本 磨由子, 國島 伸治, 松下 正
  • Organizer: 第16回日本検査血液学会学術集会
  • Place of Presentation: 名古屋国際会議場 (愛知県名古屋市)
  • Year and Date: 2015-07-11
• [Presentation] A case of MYH9 disorders caused by a novel mutation (P.K74E) (2015)
  • Author(s): Takeshi Kanematsu, Nobuaki Suzuki, Mayuko Kishimoto, Tomohiro Aoki, Mika Ogawa, Yoshitoyo Kagami, Shinji Kunishima, Hitoshi Kiyoi, Tadashi Matsushita
  • Organizer: International Society Thrombosis and Hemostasis 2015 Congress
  • Place of Presentation: トロント (カナダ)
  • Year and Date: 2015-06-20
  • Int'l Joint Research
• [Presentation] 新規変異を有するMYH9異常症の1例 (2015)
  • Author(s): 兼松 毅, 鈴木 伸明, 小川 実加, 岸本 磨由子, 國島 伸治, 松下 正
  • Organizer: 第37回日本血栓止血学会学術集会
  • Place of Presentation: 甲府市総合市民会館 (山梨県甲府市)
  • Year and Date: 2015-05-21
• [Presentation] Mouse models of MYH9 disorders (2014)
  • Author(s): 鈴木伸明
  • Organizer: International Society of Thrombosis and Hemostasis
  • Place of Presentation: アメリカ合衆国 ミルウオーキー
  • Year and Date: 2014-06-23 – 2014-06-26
• [Presentation] α-Storage Pool病の1例 (2014)
  • Author(s): 兼松 毅, 岸本 磨由子, 鈴木 伸明, 國島 伸治, 松下 正
  • Organizer: 日本血栓止血学会
  • Place of Presentation: 大阪国際交流センター 大阪府大阪市
  • Year and Date: 2014-05-29 – 2014-05-31
• [Presentation] 血友病Bにおける免疫寛容療法 同一家系における2症例の検討 (2014)
  • Author(s): 鈴木 伸明, 飯塚 敦夫, 石津 洋二, 林 和彦, 石上 雅敏, 後藤 秀実, 小嶋 哲人, 松下 正
  • Organizer: 日本血栓止血学会
  • Place of Presentation: 大阪国際交流センター 大阪府大阪市
  • Year and Date: 2014-05-29 – 2014-05-31
• [Presentation] 遺伝子改変マウスを用いたsamll GTPase RhoFの機能解析 (2014)
  • Author(s): 岸本 磨由子, 松田 剛典, 簗瀬 正吾, 鈴木 伸明, 池尻 誠, 高木 明, 國島 伸治, 小嶋 哲人, 直江 知樹, 丸山 光生, 勝見 章, 松下 正
  • Organizer: 日本血栓止血学会
  • Place of Presentation: 大阪国際交流センター 大阪府大阪市
  • Year and Date: 2014-05-29 – 2014-05-31
• [Presentation] 抗VWFインヒビターを発症したVWD type3の遺伝子解析 (2014)
  • Author(s): 三田 直美, 鈴木 伸明, 岸本 磨由子, 梶浦 容子, 松本 祐之, 中村 栄男, 松下 正
  • Organizer: 日本血栓止血学会
  • Place of Presentation: 大阪国際交流センター 大阪府大阪市
  • Year and Date: 2014-05-29 – 2014-05-31