| Project/Area Number |
26860730
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Hematology
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| Research Institution | Kyoto University |
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Principal Investigator |
Kataoka Keisuke 京都大学, 医学(系)研究科(研究院), 特定助教 (90631383)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 骨髄異形成症候群 / 急性骨髄性白血病 / 遺伝子改変マウス / U2AF1 / 癌 / 内科 / 血液腫瘍学 / マウス |
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| Outline of Final Research Achievements |
To examine the role of U2AF1 S34F mutations, which are frequently observed in myelodysplastic syndrome, I analyzed U2af1 S34F mutation conditional knock-in mice. At first, I created a conditional knock-in mouse model using FLEX switch system. After being crossed with Vav1-cre or Mx-cre mice, this mouse model did not show sufficient Cre-mediated recombination nor expression of U2af1-mutated allele. In addition, I did not find any differences in phenotypes of hematopoietic cells between wild-type and knock-in mice. Therefore, next, I created another conditional knock-in mouse model harboring loxP-STOP cassette-loxP U2af1 S34F allele. These mice are currently under investigation.
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