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The impact of HTLV-1 infection in inflammatory response of rheumatoid arthritis

Research Project

Project/Area Number 26860754
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Collagenous pathology/Allergology
Research InstitutionUniversity of Miyazaki

Principal Investigator

Umekita Kunihiko  宮崎大学, 医学部, 講師 (20506084)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords関節リウマチ / HTLV-1 / ヒトT細胞性白血病ウイルス / HTLV-1感染 / 自己免疫疾患 / 抗サイトカイン療法 / HTLV-1感染
Outline of Final Research Achievements

The aim of this project is to investigate the impact of HTLV-1 infection in worsening mechanisms of rheumatoid arthritis (RA). At first, we evaluated cytokine profiles in HTLV-1 positive RA patients and negative RA patients. The level of IL-6 and CCL20 was higher in HTLV-1 positive RA patients than in negative RA patients. Next, to clarify the interaction between HTLV-1 infected cells and RA synovial fibroblasts (RASF), HTLV-1 infected or non-infected cell-lines were co-cultured with RASF. The expression of IL-6, CCL20, and MMP-3 mRNA in RASF was increased by co-cultured with HTLV-1 infected cells. We focused on exosomes derived from HTLV-1 infected cells as inflammatory mediator. HTLV-1 infected cell derived exosomes were added to RASF, increase expression of IL-6 and CXCL10 mRNA was observed, indicating inflammatory change. Finally, we revealed that pattern recognition receptor plays an important role in exosome induced inflammatory change of RASF.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (3 results)

All 2017 2015

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (2 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] 治療抵抗性関節リウマチにおけるHTLV-1感染の影響2017

    • Author(s)
      梅北邦彦
    • Journal Title

      アレルギーの臨床

      Volume: 37 Pages: 86-91

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Presentation] Exosome Derived From HTLV-1 Infected Cell Acts As Inflammatory Mediator to Rheumatoid Arthritis Synovial Fibroblast.2017

    • Author(s)
      Kunihiko Umekita
    • Organizer
      第18回国際ヒトレトロウイルスHTLV会議
    • Place of Presentation
      ホテルグランドアーク半蔵門(東京)
    • Year and Date
      2017-03-07
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] IL-6 May Have an Important Role in the Resistance to Anti-TNF Therapies of Human T-Lymphotropic Virus Type 1 (HTLV-1) Positive Rheumatoid Arthritis (RA) Patients; HTLV-1 Infected Cells Activate the Inflammatory Responses of RA Synovial Fibroblasts.2015

    • Author(s)
      Kunihiko Umekita
    • Organizer
      2015 ACR/ARHP Annual Meeting
    • Place of Presentation
      San Francisco
    • Year and Date
      2015-11-06
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research

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Published: 2014-04-04   Modified: 2018-03-22  

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