| Project/Area Number |
26860754
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Collagenous pathology/Allergology
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| Research Institution | University of Miyazaki |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 関節リウマチ / HTLV-1 / ヒトT細胞性白血病ウイルス / ＨＴＬＶ－１感染 / 自己免疫疾患 / 抗サイトカイン療法 / HTLV-1感染 |
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| Outline of Final Research Achievements |
The aim of this project is to investigate the impact of HTLV-1 infection in worsening mechanisms of rheumatoid arthritis (RA). At first, we evaluated cytokine profiles in HTLV-1 positive RA patients and negative RA patients. The level of IL-6 and CCL20 was higher in HTLV-1 positive RA patients than in negative RA patients. Next, to clarify the interaction between HTLV-1 infected cells and RA synovial fibroblasts (RASF), HTLV-1 infected or non-infected cell-lines were co-cultured with RASF. The expression of IL-6, CCL20, and MMP-3 mRNA in RASF was increased by co-cultured with HTLV-1 infected cells. We focused on exosomes derived from HTLV-1 infected cells as inflammatory mediator. HTLV-1 infected cell derived exosomes were added to RASF, increase expression of IL-6 and CXCL10 mRNA was observed, indicating inflammatory change. Finally, we revealed that pattern recognition receptor plays an important role in exosome induced inflammatory change of RASF.
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