• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of novel therapeutics targeting HMGA2 gene.

Research Project

Project/Area Number 26860819
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Pediatrics
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

MIYACHI Mitsuru  京都府立医科大学, 医学(系)研究科(研究院), 助教 (40584983)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords横紋筋肉腫 / HMGA2 / netropsin / がん遺伝子
Outline of Final Research Achievements

HMGA2 is a high mobility group protein, acting as a transcriptional regulator. It is highly expressed in embryonal rhabdomyosarcoma (ERMS). Here, we analyzed the function of HMGA2 in ERMS. Knockdown of HMGA2 resulted in cell growth suppression, G1 phase cell cycle arrest, and muscle differentiation in the ERMS cell lines. HMGA2-expressing mouse myoblast cell lines formed larger tumors than control cells did. On the other hand, tumors induced by HMGA2-depleted ERMS cell lines were smaller than those induced by control cells. Next, we examined the effect of the HMGA2 inhibitor netropsin, which competitively inhibits DNA binding of HMGA2. The IC50s of netropsin in RD, RMS-YM and Rh18 cells were 148, 158 and 87 microM, respectively. Netropsin treatment reduced the size of xenograft tumors of ERMS cells. These results suggests HMGA2 plays an oncogenic role in ERMS cell lines, and netropsin has anti-tumor effects.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (1 results)

All 2014

All Presentation (1 results)

  • [Presentation] Functional analysis of HMGA2 expression in fusion-negative rhabdomyosaroma and examination of antitumor effect of netroptin2014

    • Author(s)
      大内 一孝、宮地 充、桒原 康通、土屋 邦彦、家原 知子、細井 創
    • Organizer
      第56回日本小児血液・がん学会学術集会
    • Place of Presentation
      岡山コンベンションセンター
    • Year and Date
      2014-11-28 – 2014-11-30
    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2017-05-10  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi