| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
In this study, we investigated the role of microRNAs in the pathogenesis of dermatofibrosacoma protuberans (DFSP). MicroRNA PCR array showed the expression of miR-205 was down-regulated in DFSP compared with dermatofibroma and normal skin. miR-205 inhibitor increased cell proliferation and low-density lipoprotein receptorrelated protein-1 (LRP-1) protein in cultured normal dermal fibroblasts. Knockdown of LRP-1 suppressed cell growth and down-regulated extracellular signal-regulated kinase (ERK) phosphorylation in cultured DFSP cells. Taken together, LRP-1 overexpression caused by the miR-205 down-regulation may play a role in the abnormal proliferation of DFSP cells via directly regulating ERK phosphorylation.
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