2015 Fiscal Year Final Research Report
The role of microRNAs in the pathogenesis of dermatofibrosarcoma protuberans.
Project/Area Number |
26860886
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Dermatology
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Research Institution | Kumamoto University |
Principal Investigator |
Kajihara Ikko 熊本大学, 大学院生命科学研究部, 特任助教 (90433036)
|
Project Period (FY) |
2014-04-01 – 2016-03-31
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Keywords | 隆起性皮膚線維肉腫 / miR-205 / LRP-1 |
Outline of Final Research Achievements |
In this study, we investigated the role of microRNAs in the pathogenesis of dermatofibrosacoma protuberans (DFSP). MicroRNA PCR array showed the expression of miR-205 was down-regulated in DFSP compared with dermatofibroma and normal skin. miR-205 inhibitor increased cell proliferation and low-density lipoprotein receptorrelated protein-1 (LRP-1) protein in cultured normal dermal fibroblasts. Knockdown of LRP-1 suppressed cell growth and down-regulated extracellular signal-regulated kinase (ERK) phosphorylation in cultured DFSP cells. Taken together, LRP-1 overexpression caused by the miR-205 down-regulation may play a role in the abnormal proliferation of DFSP cells via directly regulating ERK phosphorylation.
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Free Research Field |
皮膚科学
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