| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Three gastric cancer cell lines (MKN1, KATO-III and MKN-45) were injected into the gastric wall of BALB/c-nu and NOD-scid mice. However, we could not find any primary tumor on gastric wall. To establish a mouse model with reproducible development of liver metastasis we used NOD-scid mice and 1x10 to the power of 6 (MKN1) were injected via portal vein. All mice developed liver metastasis. However, none of the mice had detectable Circulating Tumor Cells (CTC).
We could not established an orthotopic mouse model of gastric cancer which reliably develops CTC.
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