The identification and functional analysis of micro RNA via polycomb proteins in HCC progression.

• Project/Area Number: 26861050
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General surgery
• Research Institution: Kumamoto University
• Principal Investigator: MASUDA Toshiro 熊本大学, 医学部附属病院, 非常勤診療医師 (50551256)
• Research Collaborator: NAKAGAWA Shigeki 熊本大学, 医学部附属病院, 非常勤診療医師 (10594872)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: HCC / micro RNA / EZH2 / Cancer progression / 肝細胞癌 / マイクロRNA / 細胞周期 / Zeste Homolog 2

Outline of Final Research Achievements

Previously we reported that the EZH2 (Enhancer of Zeste Homolog 2) regulate cancer progression. Here, we retrieved microRNAs which regulate the EZH2 expression from the database of HCC (Hepatocellular carcinoma) cell lines, and we identified miR-32 and miR-382. We analyzed how miR-EZH2 pathway worked in HCC progression, and we found that the pathway suppressed p16INK4a and CDH1. p16INK4a is known as tumor suppressor gene and regulate cell cycle and senescence, and CDH1 is known as a EMT (Epithelial-Mesenchymal Transition) marker and suppress EMT.

Research Products

• [Journal Article] Intraoperative baseline oxygen consumption as a prognostic factor in emergency open abdominal surgery. (2016)
  • Author(s): Masuda T, Kuramoto M, Tanimoto H, Yamamoto K, Ikeshima S, Kitano Y, Kuroda D, Shimada S, Baba H.
  • Journal Title: J Crit Care Volume: 32 Pages: 42-7
  • DOI: 10.1016/j.jcrc.2015.11.014
  • Peer Reviewed
• [Journal Article] The effect of extensive intraoperative peritoneal lavage therapy (EIPL) on stage III B + C and cytology-positive gastric cancer patients. (2016)
  • Author(s): Masuda T, Kuramoto M, Shimada S, Ikeshima S, Yamamoto K, Nakamura K, Yoshimatsu S, Urata M,Baba H.
  • Journal Title: Int J Clin Oncol. Volume: 21(2) Issue: 2 Pages: 289-94
  • DOI: 10.1007/s10147-015-0892-6
  • Peer Reviewed
• [Journal Article] Predictive factors of pathological vascular invasion in hepatocellular carcinoma within 3 cm and 3 nodules without radiological vascular invasion. (2015)
  • Author(s): Masuda T, Beppu T, Okabe H, Nitta H, Imai K, Hayashi H, Chikamoto A, Yamamoto K, Ikeshima S, Kuramoto M, Shimada S, Baba H.
  • Journal Title: Hepatol Res. Volume: - Issue: 10 Pages: 985-991
  • DOI: 10.1111/hepr.12637
  • Peer Reviewed
• [Journal Article] Repeated hepatic resections and radio-frequency ablations may improve the survival of adult undifferentiated embryonal sarcoma of the liver: report of two cases. (2015)
  • Author(s): Masuda T, Beppu T, Doi K, Miyata T, Nakagawa S, Okabe H, Hayashi H, Ishiko T, Iyama K, Baba H.
  • Journal Title: Surg Case Rep Volume: 1(1) Issue: 1 Pages: 55-55
  • DOI: 10.1186/s40792-015-0056-y
  • Peer Reviewed
• [Journal Article] Angiopoietin-like protein 2 renders colorectal cancer cells resistant to chemotherapy by activating spleen tyrosine kinase-phosphoinositide 3-kinase-dependent anti-apoptotic signaling. (2014)
  • Author(s): Horiguchi H, Endo M, Miyamoto Y, Sakamoto Y, Odagiri H, Masuda T, Kadomatsu T, Tanoue H, Motokawa I, Terada K, Morioka MS, Manabe I, Baba H, Oike Y.
  • Journal Title: Cancer Science Volume: 105 Issue: 12 Pages: 1550-1559
  • DOI: 10.1111/cas.12554
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Splanchnicectomy for pancreatic cancer pain (2014)
  • Author(s): Masuda T, Kuramoto M, Shimada S, Ikeshima S, Yamamoto K, Nakamura K, Baba H
  • Journal Title: BioMed Res Int Volume: 2014 Pages: 941726-941726
  • DOI: 10.1155/2014/941726
  • Peer Reviewed
• [Journal Article] Sarcopenia is a prognostic factor in living donor liver transplantation. (2014)
  • Author(s): Masuda T, Shirabe K, Ikegami T, Harimoto N, Yoshizumi T, 他
  • Journal Title: Liver Transplantation Volume: 20 Issue: 4 Pages: 401-407
  • DOI: 10.1002/lt.23811
  • Peer Reviewed