| Project/Area Number |
26861111
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cardiovascular surgery
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | shugoshin / 動脈硬化 / 老化 / ノックアウトマウス / 血管 |
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| Outline of Final Research Achievements |
Shugoshin is an essential protein in mitosis and meiosis, and it has an important role to protect chromosome cohesion at centromeres. Without Shugoshin, chromosome separations would be failed, and it would be lethal or develop cancers. Therefore, we hypothesized chromosomal instabilities because of lack of Shugoshin in vascular cells would lead to vascular diseases, and we tried to create vascular endothelial cells and smooth muscle cells specific Shugoshin conditional knock-out mice. We got more than 20 mice births, however, vascular cells specific Shugoshin conditional knock-out mouse was not gained. Vascular cells specific Shugoshin conditional knock-out mice may be embryonic lethal. Then, we investigated vascular phenotypes of vascular cells specific Shugoshin conditional hetero knock-out mice, however, there were not significant phenotypes. The current study revealed vascular cells specific Shugoshin knock-out mice were embryonic lethal.
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